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Literature summary extracted from
- Molecular and biochemical characterization of D-phosphoglycerate dehydrogenase from Entamoeba histolytica. A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways (2004), Eur. J. Biochem., 271, 2670-2681.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.95 | expression in Escherichia coli | Entamoeba histolytica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.95 | 3-phosphohydroxypyruvate | substrate inhibition | Entamoeba histolytica |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.95 | 0.015 | - |
phosphohydroxypyruvate | pH 6.5 | Entamoeba histolytica | |
| 1.1.1.95 | 0.0177 | - |
NADH | pH 6.5 | Entamoeba histolytica | |
| 1.1.1.95 | 0.0867 | - |
NAD+ | pH 9.0 | Entamoeba histolytica | |
| 1.1.1.95 | 0.141 | - |
NADPH | pH 6.5 | Entamoeba histolytica | |
| 1.1.1.95 | 0.212 | - |
3-phosphoglycerate | pH 9.0 | Entamoeba histolytica | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.95 | KCl | optimal salt concentration is 350-400 mM | Entamoeba histolytica | |
| 1.1.1.95 | NaCl | optimal salt concentration is 350-400 mM | Entamoeba histolytica | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.95 | 33500 | - |
2 * 33500, calculation from nucleotide sequence | Entamoeba histolytica |
| 1.1.1.95 | 70000 | 74000 | gel filtration | Entamoeba histolytica |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.95 | Entamoeba histolytica | Q76KF5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.95 | - |
Entamoeba histolytica |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.95 | -80°C, 50% glycerol, purified recombinant enzyme remains active after 1 month of storage | Entamoeba histolytica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.95 | 3-phospho-D-glycerate + NADP+ | - |
Entamoeba histolytica | 3-phosphohydroxypyruvate + NADPH + H+ | - |
? | |
| 1.1.1.95 | 3-phosphoglycerate + NAD+ | - |
Entamoeba histolytica | 3-phosphohydroxypyruvate + NADH | - |
r | |
| 1.1.1.95 | additional information | no activity with 2-oxoglutarate | Entamoeba histolytica | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.95 | dimer | 2 * 33500, calculation from nucleotide sequence | Entamoeba histolytica |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.95 | D-phosphoglycerate dehydrogenase | - |
Entamoeba histolytica |
| 1.1.1.95 | EhPGDH | - |
Entamoeba histolytica |
| 1.1.1.95 | PGDH | - |
Entamoeba histolytica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.95 | 6 | 6.5 | reaction with 3-phosphohydroxypyruvate + NADH | Entamoeba histolytica |
| 1.1.1.95 | 9 | - |
reaction with 3-phosphoglycerate and NAD+ | Entamoeba histolytica |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.95 | NADH | - |
Entamoeba histolytica | |
| 1.1.1.95 | NADPH | less efficiently than NADH | Entamoeba histolytica | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.1.1.95 | Entamoeba histolytica | calculation from nucleotide sequence | - |
6.9 |
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