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Literature summary extracted from

  • Seah, S.Y.; Britton, K.L.; Rice, D.W.; Asano, Y.; Engel, P.C.
    Kinetic analysis of phenylalanine dehydrogenase mutants designed for aliphatic amino acid dehydrogenase activity with guidance from homology-based modelling (2003), Eur. J. Biochem., 270, 4628-4634.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.20 expression in Escherichia coli Lysinibacillus sphaericus

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.20 G124A 12% of wild-type kcat with L-phenylalanine Lysinibacillus sphaericus
1.4.1.20 G124A/L307V 7.5% of wild-type kcat with L-phenylalanine Lysinibacillus sphaericus
1.4.1.20 L307V 26% of wild-type kcat with L-phenylalanine Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.20 39000
-
 10 * 39000, SDS_PAGE Lysinibacillus sphaericus
1.4.1.20 380000
-
 gel filtration Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.20 L-phenylalanine + H2O + NAD+ Lysinibacillus sphaericus
-
 phenylpyruvate + NH3 + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.20 recombinant PheDH Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 L-isoleucine + H2O + NAD+
-
 Lysinibacillus sphaericus 3-methyl-2-oxopentanoate + NH3 + NADH
-
 r
1.4.1.20 L-leucine + H2O + NAD+
-
 Lysinibacillus sphaericus 3,3-dimethyl-2-oxo-butanoate + NH3 + NADH
-
 r
1.4.1.20 L-methionine + H2O + NAD+
-
 Lysinibacillus sphaericus (4-methylsulfanyl)-2-oxobutanoate + NH3 + NADH
-
 r
1.4.1.20 L-norleucine + H2O + NAD+
-
 Lysinibacillus sphaericus 2-oxohexanoate + NH3 + NADH
-
 r
1.4.1.20 L-norvaline + H2O + NAD+
-
 Lysinibacillus sphaericus 2-oxopentanoate + NH3 + NADH
-
 r
1.4.1.20 L-phenylalanine + H2O + NAD+
-
 Lysinibacillus sphaericus phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-tyrosine + H2O + NAD+
-
 Lysinibacillus sphaericus (4-hydroxyphenyl)pyruvate + NH3 + NADH
-
 r
1.4.1.20 L-valine + H2O + NAD+
-
 Lysinibacillus sphaericus 3-methyl-2-oxobutanoate + NH3 + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.20 decamer 10 * 39000, SDS_PAGE Lysinibacillus sphaericus

Synonyms

EC Number Synonyms Comment Organism
1.4.1.20 PheDH
-
 Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 NAD+
-
 Lysinibacillus sphaericus
1.4.1.20 NADH
-
 Lysinibacillus sphaericus