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Literature summary extracted from

  • Kukulski, F.; Komoszynski, M.
    Purification and characterization of NTPDase1 (ecto-apyrase) and NTPDase2 (ecto-ATPase) from porcine brain cortex synaptosomes (2003), Eur. J. Biochem., 270, 3447-3454.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 1-hydroxy-naphthalene-3,6-disulfonic acid
-
 Sus scrofa
3.6.1.5 Ba2+
-
 Sus scrofa
3.6.1.5 Cu2+
-
 Sus scrofa
3.6.1.5 EDTA about 40% inhibition at 5 mM Sus scrofa
3.6.1.5 Sodium azide
-
 Sus scrofa
3.6.1.5 suramin
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 0.095
-
 ADP pH 7.6, 37°C Sus scrofa
3.6.1.5 0.097
-
 ATP pH 7.6, 37°C Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+
-
 Sus scrofa
3.6.1.5 Mg2+ most effective as activating cation, but not absolutely required Sus scrofa
3.6.1.5 Mn2+ activation Sus scrofa
3.6.1.5 additional information no effect: Na+ at 100 mM or K+ at 4 mM Sus scrofa
3.6.1.5 Zn2+
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.5 72000
-
 x * 72000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.5 additional information Sus scrofa enzyme terminates P2 receptor-mediated signal transmission ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.5
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.5 neuronal cell synaptosome from brain cortex Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.5 1.3
-
 pH 7.6, 37°C, presence of 1.5 mM Cu2+ Sus scrofa
3.6.1.5 4.1
-
 pH 7.6, 37°C, presence of 1.5 mM Ba2+ Sus scrofa
3.6.1.5 10.8
-
 pH 7.6, 37°C, presence of 5 mM EDTA Sus scrofa
3.6.1.5 19.4
-
 pH 7.6, 37°C, presence of 1.5 mM Zn2+ Sus scrofa
3.6.1.5 25.6
-
 pH 7.6, 37°C Sus scrofa
3.6.1.5 69.4
-
 pH 7.6, 37°C, presence of 1.5 mM Ca2+ Sus scrofa
3.6.1.5 84.2
-
 pH 7.6, 37°C, presence of 1.5 mM Mn2+ Sus scrofa
3.6.1.5 94.7
-
 pH 7.6, 37°C, presence of 1.5 mM Mg2+ Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ADP + H2O 71% of activity compared to ATP Sus scrofa AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O
-
 Sus scrofa AMP + 2 phosphate
-
 ?
3.6.1.5 CDP + H2O 42% of activity compared to ATP Sus scrofa CMP + phosphate
-
 ?
3.6.1.5 CTP + 2 H2O 87% of activity compared to ATP Sus scrofa CMP + 2 phosphate
-
 ?
3.6.1.5 GDP + H2O 61% of activity compared to ATP Sus scrofa GMP + phosphate
-
 ?
3.6.1.5 GTP + 2 H2O 91% of activity compared to ATP Sus scrofa GMP + 2 phosphate
-
 ?
3.6.1.5 ITP + 2 H2O 78% of activity compared to ATP Sus scrofa IMP + 2 phosphate
-
 ?
3.6.1.5 additional information enzyme terminates P2 receptor-mediated signal transmission Sus scrofa ?
-
 ?
3.6.1.5 TDP + H2O 44% of activity compared to ATP Sus scrofa TMP + phosphate
-
 ?
3.6.1.5 TTP + 2 H2O 82% of activity compared to ATP Sus scrofa TMP + 2 phosphate
-
 ?
3.6.1.5 UDP + H2O 56% of activity compared to ATP Sus scrofa UMP + phosphate
-
 ?
3.6.1.5 UTP + 2 H2O 94% of activity compared to ATP Sus scrofa UMP + 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.5 ? x * 72000, SDS-PAGE Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.5 860
-
 ATP pH 7.6, 37°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.5 7.6
-
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.5 0.07
-
 1-hydroxy-naphthalene-3,6-disulfonic acid pH 7.6, 37°C, substrate ADP Sus scrofa
3.6.1.5 0.103
-
 1-hydroxy-naphthalene-3,6-disulfonic acid pH 7.6, 37°C, substrate ATP Sus scrofa
3.6.1.5 1.7
-
 suramin pH 7.6, 37°C, substrate ADP Sus scrofa
3.6.1.5 1.8
-
 suramin pH 7.6, 37°C, substrate ATP Sus scrofa
3.6.1.5 5
-
 Sodium azide pH 7.6, 37°C, substrate ADP Sus scrofa
3.6.1.5 12
-
 Sodium azide pH 7.6, 37°C, substrate ATP Sus scrofa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.5 Sus scrofa isoelectric focusing
-
 4.9