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Literature summary extracted from

  • Curir, P.; Lanzotti, V.; Dolci, M.; Dolci, P.; Pasini, C.; Tollin, G.
    Purification and properties of a new S-adenosyl-L-methionine:flavonoid 4'-O-methyltransferase from carnation (Dianthus caryophyllus L.) (2003), Eur. J. Biochem., 270, 3422-3431.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.155 additional information activity is increased, in both in vitro and in vivo carnation tissues, by the inoculation with Fusarium oxysporum f. sp.dianthi Dianthus caryophyllus
2.1.1.231 additional information activity is increased, in both in vitro and in vivo carnation tissues, by the inoculation with Fusarium oxysporum f. sp. dianthi Dianthus caryophyllus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.155 Ca2+ 10 mM, 19% inhibition Dianthus caryophyllus
2.1.1.155 iodoacetamide 1 mM, 60% inhibition Dianthus caryophyllus
2.1.1.155 Mg2+ 10 mM, 27% inhibition Dianthus caryophyllus
2.1.1.155 Mn2+ 1 mM: 21% inhibition, 10 mM: 57% inhibition Dianthus caryophyllus
2.1.1.155 Phenylmercuriacetate 1 mM, 80% inhibition Dianthus caryophyllus
2.1.1.155 S-adenosyl-L-homocysteine competitive, inhibition kinetics Dianthus caryophyllus
2.1.1.231 iodoacetamide 1 mM, 40% residual activity Dianthus caryophyllus
2.1.1.231 Mn2+ 1 mM, 79% residual activity Dianthus caryophyllus
2.1.1.231 phenylmercuric acetate 1 mM, 20% residual activity Dianthus caryophyllus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.155 additional information
-
additional information kinetic mechanism Dianthus caryophyllus
2.1.1.155 0.0017
-
kaempferol pH 7, 25°C Dianthus caryophyllus
2.1.1.155 0.0033
-
apigenin pH 7, 25°C Dianthus caryophyllus
2.1.1.155 0.011
-
4'-hydroxyflavanone pH 7, 25°C Dianthus caryophyllus
2.1.1.155 0.0735
-
genistein pH 7, 25°C Dianthus caryophyllus
2.1.1.231 0.0017
-
kaempferol pH 7.0, 25°C Dianthus caryophyllus
2.1.1.231 0.0033
-
apigenin pH 7.0, 25°C Dianthus caryophyllus
2.1.1.231 0.011
-
4'-hydroxyflavanone pH 7.0, 25°C Dianthus caryophyllus
2.1.1.231 0.0735
-
genistein pH 7.0, 25°C Dianthus caryophyllus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.155 additional information no divalent metal ion requirement Dianthus caryophyllus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.155 43000 45000 gel filtration, native PAGE Dianthus caryophyllus
2.1.1.231 43000 45000 gel filtration and PAGE Dianthus caryophyllus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.155 additional information Dianthus caryophyllus the role of F 4’-OMT is mainly defensive against parasites, rather than structural ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.155 Dianthus caryophyllus
-
cv. Novada
-
2.1.1.231 Dianthus caryophyllus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.155 1399fold Dianthus caryophyllus
2.1.1.231
-
Dianthus caryophyllus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.155 S-adenosyl-L-methionine + kaempferol = S-adenosyl-L-homocysteine + kaempferide the enzyme acts on the hydroxy group in the 4'-position of some flavones, flavanones and isoflavones, kaempferol, apigenin and kaempferol triglucoside are substrates, as is genistein, which reacts more slowly, compounds with a hydroxy group in the 3' and 4' positions, such as quercetin and eriodictyol, do not act as substrates, similar to EC 2.1.1.75, apigenin 4'-O-methyltransferase and EC 2.1.1.83, 3,7-dimethylquercitin 4'-O-methyltransferase, ping-pong mechanism excluding the formation of a ternary complex Dianthus caryophyllus
2.1.1.231 S-adenosyl-L-methionine + a 4'-hydroxyflavanone = S-adenosyl-L-homocysteine + a 4'-methoxyflavanone kinetic analysis indicates a Ping-Pong mechanism and excludes the formation of a ternary complex Dianthus caryophyllus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.155 callus
-
Dianthus caryophyllus
-
2.1.1.155 stem
-
Dianthus caryophyllus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.155 70.5
-
pH 7, 25°C Dianthus caryophyllus
2.1.1.231 19590
-
pH 7.0, 25°C Dianthus caryophyllus

Storage Stability

EC Number Storage Stability Organism
2.1.1.155 -20°C, 2 weeks, 50% loss of activity Dianthus caryophyllus
2.1.1.231 -20°C, two weeks, about 50% loss of activity Dianthus caryophyllus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.155 additional information the role of F 4’-OMT is mainly defensive against parasites, rather than structural Dianthus caryophyllus ?
-
?
2.1.1.155 additional information not: 4-hydroxybenzoic acid, gallic acid, p-coumaric acid, caffeic acid, caffeoyl-CoA, quercetin, rutin, luteolin, eriodictyol, 3’,4’,7’-trihydroxyisoflavone, datiscetin Dianthus caryophyllus ?
-
?
2.1.1.155 S-adenosyl-L-methionine + 4'-hydroxyflavanone
-
Dianthus caryophyllus S-adenosyl-L-homocysteine + 4'-methoxyflavanone
-
?
2.1.1.155 S-adenosyl-L-methionine + a flavonoid F 4’-OMT specifically methylates the hydroxy substituent in 4’-position of the flavones, flavanones and isoflavones in the presence of S-adenosyl-L-methionine, enzyme affinity for the substrate and catalytic efficiency decreases in the following order: 4’-hydroxyflavones, 4’-hydroxyflavanones, 4’-hydroxyisoflavones, ping-pong mechanism, which excludes the formation of a ternary complex Dianthus caryophyllus S-adenosyl-L-homocysteine + a 4'-methoxyflavonoid
-
?
2.1.1.155 S-adenosyl-L-methionine + apigenin
-
Dianthus caryophyllus S-adenosyl-L-homocysteine + acacetin 4’-methoxyapigenin ?
2.1.1.155 S-adenosyl-L-methionine + genistein
-
Dianthus caryophyllus S-adenosyl-L-homocysteine + biochanin A 4’-methoxygenistein ?
2.1.1.155 S-adenosyl-L-methionine + kaempferol
-
Dianthus caryophyllus S-adenosyl-L-homocysteine + kaempferide
-
?
2.1.1.155 S-adenosyl-L-methionine + kaempferol 3-O-beta-D-glucopyranosyl-1,4-O-alpha-L-rhamnopyranosyl-1,2-beta-D-glucopyranoside
-
Dianthus caryophyllus S-adenosyl-L-homocysteine + 4'-O-methylkaempferol 3-O-beta-D-glucopyranosyl-1,4-O-alpha-L-rhamnopyranosyl-1,2-beta-D-glucopyranoside
-
?
2.1.1.231 4'-hydroxyflavanone + S-adenosyl-L-methionine
-
Dianthus caryophyllus 4'-methoxyflavanone + S-adenosyl-L-homocysteine
-
?
2.1.1.231 apigenin + S-adenosyl-L-methionine
-
Dianthus caryophyllus acacetin + S-adenosyl-L-homocysteine
-
?
2.1.1.231 genistein + S-adenosyl-L-methionine
-
Dianthus caryophyllus biochanin A + S-adenosyl-L-homocysteine
-
?
2.1.1.231 kaempferol + S-adenosyl-L-methionine
-
Dianthus caryophyllus kaempferide + S-adenosyl-L-homocysteine
-
?
2.1.1.231 additional information enzyme specifically methylates the hydroxy substituent in 4'-position of the flavones, flavanones and isoflavones in the presence of S-adenosyl-L-methionine. No activity towards hydroxycinnamic acid derivatives Dianthus caryophyllus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.155 monomer
-
Dianthus caryophyllus
2.1.1.231 monomer 1 * 43000-45000, SDS-PAGE Dianthus caryophyllus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.155 F 4’-OMT
-
Dianthus caryophyllus
2.1.1.155 flavonoid methyltransferase
-
Dianthus caryophyllus
2.1.1.155 flavonoid O-methyltransferase
-
Dianthus caryophyllus
2.1.1.155 methyltransferase, flavonoid
-
Dianthus caryophyllus
2.1.1.155 S-adenosyl-L-methionine:flavonoid 4’-O-methyltransferase
-
Dianthus caryophyllus
2.1.1.231 F 4'-OMT
-
Dianthus caryophyllus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.155 25
-
assay at Dianthus caryophyllus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.155 6.9 7
-
Dianthus caryophyllus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.155 5 8.5 50% of maximum activity at pH 5.5 and 8.5, 4% of maximum activity at pH 5 Dianthus caryophyllus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.155 5 8.5 50% of maximum activity at pH 5.5 and 8.5, 4% of maximum activity at pH 5 Dianthus caryophyllus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.155 0.012
-
S-adenosyl-L-homocysteine pH 7, 25°C Dianthus caryophyllus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.155 Dianthus caryophyllus isoelectric focusing, pH-gradient: 3-9
-
4.2
2.1.1.231 Dianthus caryophyllus isoelectric focusing
-
4.2