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Literature summary extracted from

  • Sanchez, M.M.; Pastor, F.I.; Diaz, P.
    Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp. BP-23. A unique type of cellulase among Bacillales (2003), Eur. J. Biochem., 270, 2913-2919.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.176
-
 Paenibacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.176 118000
-
 calculated from amino acid sequence Paenibacillus sp.
3.2.1.176 122000
-
 SDS-PAGE Paenibacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Paenibacillus sp. Q8KKF7
-
-
3.2.1.176 Paenibacillus sp. BP-23 Q8KKF7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.176 homogeneity Paenibacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.176 0.00188
-
 bacterial microcrystalline cellulose as substrate Paenibacillus sp.
3.2.1.176 0.00488
-
 acid swollen cellulose as substrate Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-nitrophenyl cellopentaoside + H2O
-
 Paenibacillus sp. 4-nitrophenyl-cellobioside + cellotriose
-
 ?
3.2.1.176 4-nitrophenyl cellopentaoside + H2O
-
 Paenibacillus sp. BP-23 4-nitrophenyl-cellobioside + cellotriose
-
 ?
3.2.1.176 4-nitrophenyl cellotetraoside + H2O
-
 Paenibacillus sp. 4-nitrophenyl cellobioside + cellobiose
-
 ?
3.2.1.176 4-nitrophenyl cellotetraoside + H2O
-
 Paenibacillus sp. BP-23 4-nitrophenyl cellobioside + cellobiose
-
 ?
3.2.1.176 cellodextrin + H2O
-
 Paenibacillus sp. ?
-
 ?
3.2.1.176 cellodextrin + H2O
-
 Paenibacillus sp. BP-23 ?
-
 ?
3.2.1.176 cellulose + H2O acid-swollen cellulose, bacterial microcrystlline cellulose Paenibacillus sp. cellobiose + D-glucose + cellotriose
-
 ?
3.2.1.176 cellulose + H2O acid-swollen cellulose, bacterial microcrystlline cellulose Paenibacillus sp. BP-23 cellobiose + D-glucose + cellotriose
-
 ?
3.2.1.176 additional information no activity on carboxymethyl cellulose, cellobiose, cellotriose or 4-methylumbelliferyl alpha-D-glucoside Paenibacillus sp. ?
-
 ?
3.2.1.176 additional information no activity on carboxymethyl cellulose, cellobiose, cellotriose or 4-methylumbelliferyl alpha-D-glucoside Paenibacillus sp. BP-23 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.176 45
-
-
 Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.176 6
-
-
 Paenibacillus sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.176 Paenibacillus sp. calculated from amino acid sequence
-
 4.9