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Literature summary extracted from

  • Islam, M.K.; Miyoshi, T.; Kasuga-Aoki, H.; Isobe, T.; Arakawa, T.; Matsumoto, Y.; Tsuji, N.
    Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites (2003), Eur. J. Biochem., 270, 2814-2826.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.3.1 expressed in Escherichia coli TOP10F' Ascaris suum

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.3.1 Ca2+
-
 Ascaris suum
7.1.3.1 Imidodiphosphate slight inhibition Ascaris suum
7.1.3.1 NaF
-
 Ascaris suum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.3.1 0.117
-
 diphosphate recombinant protein, pH 7.5, 55°C Ascaris suum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.3.1 membrane type II enzyme, proton translocating form Ascaris suum 16020
-
7.1.3.1 soluble type I enzyme Ascaris suum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.3.1 Mg2+ required Ascaris lumbricoides
7.1.3.1 Mg2+ required Toxocara canis
7.1.3.1 Mg2+ required, highest activity at 5 mM, decrease of activity at higher concentrations Ascaris suum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.3.1 38770
-
 calculated from the predicted amino acid sequence after removal of the signal peptide Ascaris suum
7.1.3.1 39000
-
 SDS-PAGE Ascaris suum
7.1.3.1 40600
-
 calculated from predicted amino acid sequence of the entire ORF Ascaris suum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.3.1 diphosphate + H2O + H+[side 1] Ascaris lumbricoides important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation 2 phosphate + H+[side 2]
-
 ?
7.1.3.1 diphosphate + H2O + H+[side 1] Ascaris suum important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation 2 phosphate + H+[side 2]
-
 ?
7.1.3.1 diphosphate + H2O + H+[side 1] Toxocara canis important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation 2 phosphate + H+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.3.1 Ascaris lumbricoides
-
-
-
7.1.3.1 Ascaris suum Q86M43 soluble enzyme
-
7.1.3.1 Toxocara canis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.3.1 recombinant protein using His-tag Ascaris suum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.3.1 body wall
-
 Ascaris suum
-
7.1.3.1 gut epithelium
-
 Ascaris suum
-
7.1.3.1 ovary
-
 Ascaris suum
-
7.1.3.1 uterus
-
 Ascaris suum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.3.1 937
-
 recombinant protein, pH 7.5, 55°C Ascaris suum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.3.1 diphosphate + H2O + H+[side 1]
-
 Ascaris lumbricoides 2 phosphate + H+[side 2]
-
 ?
7.1.3.1 diphosphate + H2O + H+[side 1]
-
 Ascaris suum 2 phosphate + H+[side 2]
-
 ?
7.1.3.1 diphosphate + H2O + H+[side 1]
-
 Toxocara canis 2 phosphate + H+[side 2]
-
 ?
7.1.3.1 diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation Ascaris lumbricoides 2 phosphate + H+[side 2]
-
 ?
7.1.3.1 diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation Ascaris suum 2 phosphate + H+[side 2]
-
 ?
7.1.3.1 diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, diphosphate hydrolysis dependent proton translocation Toxocara canis 2 phosphate + H+[side 2]
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.3.1 638
-
 diphosphate recombinant protein, pH 7.5, 55°C Ascaris suum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.3.1 7 8
-
 Ascaris suum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
7.1.3.1 5 9.5
-
 Ascaris suum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
7.1.3.1 Ascaris suum calculated from predicted amino acid sequence and detected in isoelectric focusing
-
 7.1