Literature summary extracted from

Frerichs-Deeken, U.; Goldenstedt, B.; Gahl-Janssen, R.; Kappl, R.; Huttermann, J.; Fetzner, S.
Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 deltaqor pUF1 and analysis of the Qor proteins (2003), Eur. J. Biochem., 270, 1567-1577.

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.3.99.17	additional information	enzyme deletion mutant, not able to grow on quinoline	Pseudomonas putida

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.3.99.17	0.12	-	Quinoline	25°C, strain 86-1 deltaqor pUF1	Pseudomonas putida
1.3.99.17	0.18	-	Quinoline	25°C, wild-type	Pseudomonas putida

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.3.99.17	Pseudomonas putida	P72222	medium subunit; strains KT2440 pUF1, 86-1 deltaqor pUF1, growth on quinoline	-
1.3.99.17	Pseudomonas putida	P72223	small subunit; strains KT2440 pUF1, 86-1 deltaqor pUF1, growth on quinoline	-
1.3.99.17	Pseudomonas putida	P72224	large subunit; strains KT2440 pUF1, 86-1 deltaqor pUF1, growth on quinoline	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.3.99.17	additional information	-	specific activity of enzyme purified from strain KT2440 pUF1 is about 10% of the activity of enzyme purified from wild-type	Pseudomonas putida

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.3.99.17	quinoline + iodonitrotetrazolium chloride + H2O	-	Pseudomonas putida	isoquinoline-1-one + reduced iodonitrotetrazolium chloride	-	?

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.3.99.17	74	-	Quinoline	25°C, wild-type	Pseudomonas putida
1.3.99.17	85.4	-	Quinoline	25°C, strain 86-1 deltaqor pUF1	Pseudomonas putida

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.3.99.17	FAD	-	Pseudomonas putida
1.3.99.17	iron-sulfur centre	two distinct 2Fe-2S clusters	Pseudomonas putida
1.3.99.17	molybdopterin cytosine dinucleotide	-	Pseudomonas putida

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Release 2023.1 (January 2023)