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Literature summary extracted from

  • Frandsen, T.P.; Palcic, M.M.; Svensson, B.
    Substrate recognition by three family 13 yeast alpha-glucosidases. Evaluation of deoxygenated and conformationally biased isomaltosides (2002), Eur. J. Biochem., 269, 728-734.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.10 additional information
-
 additional information kinetics, transition state stabilization energy Saccharomyces cerevisiae
3.2.1.10 1.3
-
 4-nitrophenyl alpha-D-glucopyranoside pH 6.8, 30°C Saccharomyces cerevisiae
3.2.1.10 6.9
-
 isomaltose pH 6.8, 30°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Saccharomyces cerevisiae
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.10 alpha-D-glucopyranosyl-(1-4)-[alpha-D-glucopyranosyl-(1-6)]-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + H2O = alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + D-glucose stereochemistry, molecular substrate recognition mechanism, catayltic mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.10 additional information
-
 catalytic efficiency with different substrates, substrate specificity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 2'-deoxy-methyl-alpha-isomaltoside + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 2-deoxy-methyl-alpha-isomaltoside + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 3'-deoxy-methyl-alpha-isomaltoside + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 3-deoxy-methyl-alpha-isomaltoside + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 4'-deoxy-methyl-alpha-isomaltoside + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 4-deoxy-methyl-alpha-isomaltoside + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 4-nitrophenyl alpha-D-glucopyranoside
-
 Saccharomyces cerevisiae 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.10 6'-deoxy-methyl-alpha-isomaltoside + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 6(R)-ethyl-alpha-isomaltoside + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 6(S)-ethyl-alpha-isomaltoside + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 isomaltose + H2O
-
 Saccharomyces cerevisiae alpha-D-glucose + D-glucose
-
 ?
3.2.1.10 methyl-alpha-isomaltoside + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.10 additional information stereochemistry and -specificity, overview Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.10 dextrin 6-alpha-glucanohydrolase
-
 Saccharomyces cerevisiae
3.2.1.10 More enzyme belongs to alpha-glucoside hydrolase family 13 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.10 30
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.10 33.3
-
 isomaltose pH 6.8, 30°C Saccharomyces cerevisiae
3.2.1.10 129
-
 4-nitrophenyl alpha-D-glucopyranoside pH 6.8, 30°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.10 6.8
-
 assay at Saccharomyces cerevisiae