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Literature summary extracted from

  • Berrin, J.G.; McLauchlan, W.R.; Needs, P.; Williamson, G.; Puigserver, A.; Kroon, P.A.; Juge, N.
    Functional expression of human liver cytosolic beta-glucosidase in Pichia pastoris. Insights into its role in the metabolism of dietary glucosides (2002), Eur. J. Biochem., 269, 249-258.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Pichia pastoris Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside 0.001 mM, 64% inhibition after 30 min incubation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.01
-
 luteolin-4'-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.022
-
 apigenin-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.032
-
 quercetin-4'-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.035
-
 genistein-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.042
-
 quercetin-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.05
-
 luteolin-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.12
-
 daidzein-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.25
-
 eriodictyol-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.274
-
 quercetin-3,4'-di-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.37
-
 p-nitrophenyl-beta-D-fucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.43
-
 naringenin-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.57
-
 p-nitrophenyl-alpha-L-arabinopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 1.58
-
 p-nitrophenyl-beta-D-xylopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 1.76
-
 p-nitrophenyl-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 3.14
-
 p-nitrophenyl-beta-D-galactopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 3.2
-
 daidzein 7-O-(6''-O-beta-D-malonyl-beta-D-glucoside) 37°C, pH 6.5 Homo sapiens
3.2.1.21 52.6
-
 p-nitrophenyl-beta-L-arabinopyranoside 37°C, pH 6.5 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 51900
-
 1 * 51900, SDS-PAGE Homo sapiens
3.2.1.21 53000
-
 1 * 53000, SDS-PAGE, recombinant CBG Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 native CBG, cation-exchange chromatography, Octyl-Sepharose, chromatofocusing, gel filtration, recombinant CBG, Octyl-Sepharose Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 10
-
 recombinant CBG, hydrolysis of 4-nitrophenyl-beta-D-glucopyranoside Homo sapiens
3.2.1.21 12.8
-
 native CBG, hydrolysis of 4-nitrophenyl-beta-D-glucopyranoside Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 apigenin-beta-D-glucopyranoside + H2O
-
 Homo sapiens apigenin + beta-D-glucose
-
 ?
3.2.1.21 daidzein 7-O-(6-O-malonyl-beta-D-glucoside) + H2O
-
 Homo sapiens daidzein + 6-O-malonyl-beta-D-glucopyranose
-
 ?
3.2.1.21 daidzein-7-beta-D-glucopyranoside + H2O
-
 Homo sapiens daidzein + beta-d-glucose
-
 ?
3.2.1.21 eriodictyol-7-beta-D-glucopyranoside + H2O
-
 Homo sapiens eriodictyol + D-glucose
-
 ?
3.2.1.21 genistein-7-beta-D-glucopyranoside + H2O
-
 Homo sapiens genistein + beta-D-glucose
-
 ?
3.2.1.21 luteolin-3',7-dibeta-D-glucopyranoside + H2O
-
 Homo sapiens luteolin + beta-D-glucose
-
 ?
3.2.1.21 luteolin-4'-beta-D-glucopyranoside + H2O
-
 Homo sapiens luteolin + beta-D-glucose
-
 ?
3.2.1.21 luteolin-7-beta-D-glucopyranoside + H2O
-
 Homo sapiens luteolin + beta-D-glucose
-
 ?
3.2.1.21 naringenin-7-beta-D-glucopyranoside + H2O
-
 Homo sapiens naringenin + beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-alpha-L-arabinopyranoside + H2O
-
 Homo sapiens p-nitrophenol + alpha-L-arabinose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-fucopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-galactopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-xylopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-xylose
-
 ?
3.2.1.21 p-nitrophenyl-beta-L-arabinopyranoside + H2O
-
 Homo sapiens p-nitrophenol + beta-L-arabinose
-
 ?
3.2.1.21 quercetin 3,4'-O-di-beta-D-glucopyranoside + H2O
-
 Homo sapiens quercetin + 2 beta-D-glucose
-
 ?
3.2.1.21 quercetin 4'-O-beta-D-glucopyranoside + H2O
-
 Homo sapiens quercetin + beta-D-glucose
-
 ?
3.2.1.21 quercetin 7-O-beta-D-glucopyranoside + H2O
-
 Homo sapiens quercetin + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 51900, SDS-PAGE Homo sapiens
3.2.1.21 monomer 1 * 53000, SDS-PAGE, recombinant CBG Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 CBG
-
 Homo sapiens
3.2.1.21 reCBG
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 50
-
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 37
-
 less than 20% loss of activity after 24 h Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 0.24
-
 daidzein 7-O-(6''-O-beta-D-malonyl-beta-D-glucoside) 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.3
-
 quercetin-3,4'-di-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.66
-
 p-nitrophenyl-beta-L-arabinopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.69
-
 quercetin-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 0.75
-
 p-nitrophenyl-beta-D-xylopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 1.08
-
 quercetin-4'-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 1.17
-
 luteolin-4'-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 1.26
-
 eriodictyol-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 1.53
-
 genistein-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 1.53
-
 apigenin-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 2.6
-
 naringenin-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 3.05
-
 luteolin-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 3.55
-
 daidzein-7-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 5.97
-
 p-nitrophenyl-alpha-L-arabinopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 10.7
-
 p-nitrophenyl-beta-D-fucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 12.1
-
 p-nitrophenyl-beta-D-glucopyranoside 37°C, pH 6.5 Homo sapiens
3.2.1.21 17.6
-
 p-nitrophenyl-beta-D-galactopyranoside 37°C, pH 6.5 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 4
-
 less than 4% of maximal activity Homo sapiens
3.2.1.21 5 7.5 more than 70% of maximal activity Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Homo sapiens isoelectric focusing
-
 4.9