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Literature summary extracted from

  • Knowles, A.F.; Nagy, A.K.; Strobel, R.S.; Wu-Weis, M.
    Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase (2002), Eur. J. Biochem., 269, 2373-2382.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.5 NP-40 0.1%, about 3fold activation Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 azide at 10 mM and pH 7.4, 21% inhibition of ATP hydrolysis, 85% inhibition of ADP hydrolysis Gallus gallus
3.6.1.5 diphosphate at 10 mM and pH 7.4, 51% inhibition of ATP hydrolysis, 97% inhibition of ADP hydrolysis Gallus gallus
3.6.1.5 fluoride at 10 mM and pH 7.4, 2% inhibition of ATP hydrolysis, 91% inhibition of ADP hydrolysis Gallus gallus
3.6.1.5 additional information not inhibitory: Concanavalin A, suramin Gallus gallus
3.6.1.5 vanadate at 10 mM and pH 7.4, 7% inhibition of ATP hydrolysis, 54% inhibition of ADP hydrolysis Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 0.13
-
 ATP pH 6.4, 37°C Gallus gallus
3.6.1.5 0.51
-
 ATP pH 7.4, 37°C Gallus gallus
3.6.1.5 0.72
-
 ADP pH 6.4, 37°C Gallus gallus
3.6.1.5 5.3
-
 ADP pH 7.4, 37°C Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.5 membrane
-
 Gallus gallus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+ about 30% of the activity with Mg2+, at 5 mM and pH 7.4 Gallus gallus
3.6.1.5 Mg2+ best activation Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.5 85000
-
 x * 85000, SDS-PAGE Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.5
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.5 liver bile canaliculi Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.5 1242
-
 pH 7.4, 37°C Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ADP + H2O
-
 Gallus gallus AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O
-
 Gallus gallus AMP + 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.5 ? x * 85000, SDS-PAGE Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.5 6
-
 substrate ADP Gallus gallus
3.6.1.5 8
-
 substrate ATP Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.5 6 6.5 substrate ADP Gallus gallus
3.6.1.5 7.5 8.5 substrate ATP Gallus gallus