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Literature summary extracted from

  • Gerasimenko, I.; Sheludko, Y.; Ma, X.; Stockigt, J.
    Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids (2002), Eur. J. Biochem., 269, 2204-2213.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.105 fusion protein with intein tag, expressed in Escherichia coli Rauvolfia serpentina

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.105 Cu2+ 8.8% inhibition at 1 mM Rauvolfia serpentina
3.2.1.105 serpentine 25.2% inhibition at 1 mM Rauvolfia serpentina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.105 0.12
-
 strictosidine pH 5, 30°C Rauvolfia serpentina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.105 450000
-
 gel filtration Rauvolfia serpentina

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.105 Rauvolfia serpentina Q8GU20
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.105 homogeneity Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.105 18,19-dihydrostrictosidine + H2O
-
 Rauvolfia serpentina D-glucose + 18,19-dihydrostrictosidine aglycone
-
 ?
3.2.1.105 5alpha-carboxystrictosidine + H2O
-
 Rauvolfia serpentina D-glucose + 5alpha-carboxystrictosidine aglycone
-
 ?
3.2.1.105 dolichantoside + H2O
-
 Rauvolfia serpentina D-glucose + dolichantoside aglycone
-
 ?
3.2.1.105 additional information no substrates: strictosidine pentaacetate, vincoside, strictosidine lactam, vincoside lactam, raucafficrine, 1,2-dihydrocafficrine, 1-methyl-1,2-dihydrocarcafficrine, secologanin, loganin, 4-nitrophenylglucoside, arbutin, vanillin-glucoside, isatinoxim-glucoside, prunasin, rhapontin, rutin, sinigrin and zeatin-glucoside Rauvolfia serpentina ?
-
 ?
3.2.1.105 Nbeta-acetylstrictosidine + H2O weak substrate Rauvolfia serpentina D-glucose + Nbeta-acetylstrictosidine aglycone
-
 ?
3.2.1.105 Nbeta-methylstrictosidine + H2O weak substrate Rauvolfia serpentina D-glucose + Nbeta-methylstrictosidine aglycone
-
 ?
3.2.1.105 strictosidine + H2O
-
 Rauvolfia serpentina D-glucose + strictosidine aglycone
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.105 50
-
-
 Rauvolfia serpentina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.105 5.2
-
-
 Rauvolfia serpentina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.105 4.2 8 50% of maximal activity at oH 4.2, above pH 5.2 the activity slowly decreases up to pH 8 Rauvolfia serpentina

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.105 Rauvolfia serpentina calculated from amino acid sequence
-
 6