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Literature summary extracted from

  • Kanao, T.; Kawamura, M.; Fukui, T.; Atomi, H.; Imanaka, T.
    Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola. A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle (2002), Eur. J. Biochem., 269, 1926-1931.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 construction of a genomic cDNA library, gene Cl-idh, DNA sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Chlorobium limicola

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 additional information no inhibition of carboxylation by citrate, pyruvate, succinate, fumarate, malate, glyoxylate, ATP, and ADP Chlorobium limicola
1.1.1.42 oxaloacetate competitive, inhibits the reverse reaction, over 50% inhibition at 1 mM, synergistically with glyoxylate Chlorobium limicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.027
-
 NADP+ recombinant enyme, pH 9.0 Chlorobium limicola
1.1.1.42 0.045
-
 isocitrate recombinant enyme, pH 9.0 Chlorobium limicola
1.1.1.42 1.1
-
 2-oxoglutarate recombinant enyme, pH 7.0 Chlorobium limicola
1.1.1.42 1.3
-
 CO2 recombinant enyme, pH 7.0 Chlorobium limicola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 80000
-
 native PAGE Chlorobium limicola
1.1.1.42 81000
-
 gel filtration Chlorobium limicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Chlorobium limicola carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle, preference for carboxylation reaction direction 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Chlorobium limicola
-
 green sulfur bacterium, strain M1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 recombinant enzyme from Escherichia coli by ion exchange and gel filtration to homogeneity, 1.84fold Chlorobium limicola

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.42 36
-
 purified recombinant enzyme Chlorobium limicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Chlorobium limicola 2-oxoglutarate + CO2 + NADPH + H+
-
 r
1.1.1.42 isocitrate + NADP+ carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle, preference for carboxylation reaction direction Chlorobium limicola 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.42 monomer 1 * 80000 Chlorobium limicola

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 isocitrate dehydrogenase
-
 Chlorobium limicola
1.1.1.42 NADP-dependent IDH
-
 Chlorobium limicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7
-
 reverse reaction Chlorobium limicola
1.1.1.42 9
-
 forward reaction Chlorobium limicola

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 additional information no activity with NADH Chlorobium limicola
1.1.1.42 NADP+
-
 Chlorobium limicola
1.1.1.42 NADPH
-
 Chlorobium limicola

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.42 0.35
-
 oxaloacetate recombinant enzyme, pH 7.0 Chlorobium limicola