BRENDA - Enzyme Database

Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola. A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle

Kanao, T.; Kawamura, M.; Fukui, T.; Atomi, H.; Imanaka, T.; Eur. J. Biochem. 269, 1926-1931 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
construction of a genomic cDNA library, gene Cl-idh, DNA sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Chlorobium limicola
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
additional information
no inhibition of carboxylation by citrate, pyruvate, succinate, fumarate, malate, glyoxylate, ATP, and ADP
Chlorobium limicola
1.1.1.42
oxaloacetate
competitive, inhibits the reverse reaction, over 50% inhibition at 1 mM, synergistically with glyoxylate
Chlorobium limicola
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.027
-
NADP+
recombinant enyme, pH 9.0
Chlorobium limicola
1.1.1.42
0.045
-
isocitrate
recombinant enyme, pH 9.0
Chlorobium limicola
1.1.1.42
1.1
-
2-oxoglutarate
recombinant enyme, pH 7.0
Chlorobium limicola
1.1.1.42
1.3
-
CO2
recombinant enyme, pH 7.0
Chlorobium limicola
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
80000
-
native PAGE
Chlorobium limicola
1.1.1.42
81000
-
gel filtration
Chlorobium limicola
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Chlorobium limicola
carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle, preference for carboxylation reaction direction
2-oxoglutarate + CO2 + NADPH
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Chlorobium limicola
-
green sulfur bacterium, strain M1
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
recombinant enzyme from Escherichia coli by ion exchange and gel filtration to homogeneity, 1.84fold
Chlorobium limicola
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
36
-
purified recombinant enzyme
Chlorobium limicola
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
655430
Chlorobium limicola
2-oxoglutarate + CO2 + NADPH
-
-
-
r
1.1.1.42
isocitrate + NADP+
carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle, preference for carboxylation reaction direction
655430
Chlorobium limicola
2-oxoglutarate + CO2 + NADPH
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
monomer
1 * 80000
Chlorobium limicola
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7
-
reverse reaction
Chlorobium limicola
1.1.1.42
9
-
forward reaction
Chlorobium limicola
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
additional information
no activity with NADH
Chlorobium limicola
1.1.1.42
NADP+
-
Chlorobium limicola
1.1.1.42
NADPH
-
Chlorobium limicola
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.42
0.35
-
oxaloacetate
recombinant enzyme, pH 7.0
Chlorobium limicola
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
construction of a genomic cDNA library, gene Cl-idh, DNA sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Chlorobium limicola
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
additional information
no activity with NADH
Chlorobium limicola
1.1.1.42
NADP+
-
Chlorobium limicola
1.1.1.42
NADPH
-
Chlorobium limicola
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
additional information
no inhibition of carboxylation by citrate, pyruvate, succinate, fumarate, malate, glyoxylate, ATP, and ADP
Chlorobium limicola
1.1.1.42
oxaloacetate
competitive, inhibits the reverse reaction, over 50% inhibition at 1 mM, synergistically with glyoxylate
Chlorobium limicola
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.42
0.35
-
oxaloacetate
recombinant enzyme, pH 7.0
Chlorobium limicola
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.027
-
NADP+
recombinant enyme, pH 9.0
Chlorobium limicola
1.1.1.42
0.045
-
isocitrate
recombinant enyme, pH 9.0
Chlorobium limicola
1.1.1.42
1.1
-
2-oxoglutarate
recombinant enyme, pH 7.0
Chlorobium limicola
1.1.1.42
1.3
-
CO2
recombinant enyme, pH 7.0
Chlorobium limicola
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
80000
-
native PAGE
Chlorobium limicola
1.1.1.42
81000
-
gel filtration
Chlorobium limicola
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Chlorobium limicola
carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle, preference for carboxylation reaction direction
2-oxoglutarate + CO2 + NADPH
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
recombinant enzyme from Escherichia coli by ion exchange and gel filtration to homogeneity, 1.84fold
Chlorobium limicola
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
36
-
purified recombinant enzyme
Chlorobium limicola
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
655430
Chlorobium limicola
2-oxoglutarate + CO2 + NADPH
-
-
-
r
1.1.1.42
isocitrate + NADP+
carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle, preference for carboxylation reaction direction
655430
Chlorobium limicola
2-oxoglutarate + CO2 + NADPH
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
monomer
1 * 80000
Chlorobium limicola
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7
-
reverse reaction
Chlorobium limicola
1.1.1.42
9
-
forward reaction
Chlorobium limicola