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Literature summary extracted from

  • Kulminskaya, A.A.; Thomsen, K.K.; Shabalin, K.A.; Sidorenko, I.A.; Eneyskaya, E.V.; Savel'ev, A.N.; Neustroev, K.N.
    Isolation, enzymatic properties, and mode of action of an exo-1,3-beta-glucanase from Trichoderma viride (2001), Eur. J. Biochem., 268, 6123-6131.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.58 EDTA 1 mM, 81% of activity Trichoderma viride
3.2.1.58 Hg2+ 1 mM, 86% of activity Trichoderma viride
3.2.1.58 Zn2+ 1 mM, 83% of activity Trichoderma viride

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.58 1.9
-
 beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside pH 4.5, 37°C Trichoderma viride
3.2.1.58 2
-
 beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside pH 4.5, 37°C Trichoderma viride
3.2.1.58 2.1
-
 beta-D-glucose-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside pH 4.5, 37°C Trichoderma viride
3.2.1.58 5
-
 beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside pH 4.5, 37°C Trichoderma viride
3.2.1.58 16.5
-
 beta-D-glucose-1,3-beta-D-glucoside pH 4.5, 37°C Trichoderma viride

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.58 extracellular
-
 Trichoderma viride
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.58 Mn2+ 1 mM, 136% of activity Trichoderma viride

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.58 61000
-
 x * 61000, SDS-PAGE Trichoderma viride

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Trichoderma viride
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.58
-
 Trichoderma viride

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.58 63
-
 pH 4.5, 37°C Trichoderma viride

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
3.2.1.58 beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
3.2.1.58 beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
3.2.1.58 beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
3.2.1.58 beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
3.2.1.58 beta-D-glucose-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
3.2.1.58 curdlan + H2O
-
 Trichoderma viride ?
-
 ?
3.2.1.58 laminarin + H2O
-
 Trichoderma viride ?
-
 ?
3.2.1.58 additional information hydrolysis with inversion of the anomeric configuration, substrate subsite structure and model of enzyme binding site Trichoderma viride ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.58 ? x * 61000, SDS-PAGE Trichoderma viride
3.2.1.58 More N-terminal sequence Trichoderma viride

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 55
-
-
 Trichoderma viride

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.58 22
-
 pH 3.5-7.5, stable for 24 h Trichoderma viride

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 4.5
-
-
 Trichoderma viride