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Literature summary extracted from

  • Xia, G.; Jin, C.; Zhou, J.; Yang, S.; Zhang, S.; Jin, C.
    A novel chitinase having a unique mode of action from Aspergillus fumigatus YJ-407 (2001), Eur. J. Biochem., 268, 4079-4085.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Fe2+
-
 Aspergillus fumigatus
3.2.1.14 Hg2+
-
 Aspergillus fumigatus
3.2.1.14 Mn2+
-
 Aspergillus fumigatus
3.2.1.14 N-bromosuccinimide
-
 Aspergillus fumigatus
3.2.1.14 Pb2+
-
 Aspergillus fumigatus
3.2.1.14 Zn2+
-
 Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 46000
-
 x * 46000, SDS-PAGE Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Aspergillus fumigatus
-
 YJ-407
-
3.2.1.14 Aspergillus fumigatus YJ-407
-
 YJ-407
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Aspergillus fumigatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 3.36
-
-
 Aspergillus fumigatus

Storage Stability

EC Number Storage Stability Organism
3.2.1.14 -20 °C, lyophilized enzyme, more than 1 year without any loss in activity Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O swollen chitin, partially deacetylated chitin and lower activity towards colloidal chitin Aspergillus fumigatus ?
-
 ?
3.2.1.14 chitin + H2O swollen chitin, partially deacetylated chitin and lower activity towards colloidal chitin Aspergillus fumigatus YJ-407 ?
-
 ?
3.2.1.14 chitosan + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.14 chitosan + H2O
-
 Aspergillus fumigatus YJ-407 ?
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Aspergillus fumigatus YJ-407 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 46000, SDS-PAGE Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 60
-
-
 Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 60
-
 40% of activity after 30 min retained Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 8
-
 Aspergillus fumigatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Aspergillus fumigatus isoelectric focusing
-
 6