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Literature summary extracted from

  • Irwin, D.C.; Zhang, S.; Wilson, D.B.
    Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca (2000), Eur. J. Biochem., 267, 4988-4997.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.74 Escherichia coli and Streptomyces lividans, expression in Streptomyces lividans Thermobifida fusca
3.2.1.176 cloned into Escherichia coli and Streptomyces lividans Thermobifida fusca

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 cellobiose
-
 Thermobifida fusca

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.74 extracellular
-
 Thermobifida fusca
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.74 Thermobifida fusca Q9XCD4
-
-
3.2.1.176 Thermobifida fusca Q9XCD4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.74 no modification
-
 Thermobifida fusca
3.2.1.176 additional information no glycoprotein Thermobifida fusca

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.74 cloned Cel48A, centrifugation, phenylmethanesulfonyl fluoride and ammonium sulfate fractionation, phenyl-Sepharose column, Q-Sepharose column Thermobifida fusca
3.2.1.74 native Cel48A, HPLC Thermobifida fusca
3.2.1.176 the enzyme and catalytic domain alone are each expressed in and purified from Streptomyces lividans Thermobifida fusca

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.74 additional information
-
 cloned Cel48A, 0.068 µmol/min/µmol, filter paper, pH 5.5, at 50ºC, liquid assays Thermobifida fusca
3.2.1.74 additional information
-
 cloned Cel48A, 0.191 µmol/min/µmol, bacterial microcrystalline cellulose, pH 5.5, at 50ºC, liquid assays Thermobifida fusca
3.2.1.74 additional information
-
 cloned Cel48A, 0.288 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC, thin-layer analysis Thermobifida fusca
3.2.1.74 additional information
-
 cloned Cel48A, 0.292 µmol/min/µmol, carboxymethyl cellulose, pH 5.5, at 50ºC, liquid assays Thermobifida fusca
3.2.1.74 additional information
-
 cloned Cel48A, 0.405 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC, liquid assays Thermobifida fusca
3.2.1.74 additional information
-
 cloned Cel48A, approximately 16 µmol/min/µmol, cellotetraose, pH 5.5, at 50ºC, thin-layer analysis Thermobifida fusca
3.2.1.74 additional information
-
 cloned Cel48A, approximately 9 µmol/min/µmol, cellopentaose, pH 5.5, at 50ºC, thin-layer analysis Thermobifida fusca
3.2.1.74 additional information
-
 native Cel48A, 17.9 µmol/min/µmol, swollen cellulose, pH 5.5, at 50ºC Thermobifida fusca
3.2.1.74 additional information
-
 native Cel48A, 3.7 µmol/min/µmol, carboxymethyl cellulose, pH 5.5, at 50ºC Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.74 2,4-dinitrophenol cellobiose + H2O
-
 Thermobifida fusca ?
-
 ?
3.2.1.74 4-methylumbelliferyl beta-D-cellotrioside + H2O
-
 Thermobifida fusca 4-methylumbelliferone + cellotriose
-
 ?
3.2.1.74 4-methylumbelliferyl-beta-D-cellobioside + H2O
-
 Thermobifida fusca ?
-
 ?
3.2.1.74 bacterial microcrystalline cellulose + H2O
-
 Thermobifida fusca ?
-
 ?
3.2.1.74 carboxymethyl cellulose + H2O
-
 Thermobifida fusca ?
-
 ?
3.2.1.74 cellopentaose + H2O
-
 Thermobifida fusca ?
-
 ?
3.2.1.74 cellotetraose + H2O no activity with cellotriose and chitin Thermobifida fusca ?
-
 ?
3.2.1.74 filter paper
-
 Thermobifida fusca ?
-
 ?
3.2.1.74 swollen cellulose + H2O
-
 Thermobifida fusca cellobiose + cellotriose
-
 ?
3.2.1.176 cellopentaose + H2O
-
 Thermobifida fusca cellotriose + cellobiose
-
 ?
3.2.1.176 cellotetraose + H2O
-
 Thermobifida fusca 2 cellobiose
-
 ?
3.2.1.176 cellulose + H2O
-
 Thermobifida fusca cellobiose + ?
-
 ?
3.2.1.176 additional information Cel48A does not cleave cellotriose. No reaction at all with 4-methylumbelliferyl cellobioside and very low activity with 4-methylumbelliferyl cellotrioside Thermobifida fusca ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.74 Cel48A
-
 Thermobifida fusca
3.2.1.74 exocellulase
-
 Thermobifida fusca
3.2.1.176 Cel48A the enzyme is a family 48 exocellulase Thermobifida fusca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.74 40 50 decreases markedly above 50ºC Thermobifida fusca
3.2.1.176 60
-
 17 h, 67% loss of activity Thermobifida fusca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.74 0.00039
-
 2,4-dinitrophenol cellobiose pH 5.5 Thermobifida fusca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.74 6 6.5 50ºC Thermobifida fusca
3.2.1.176 6 6.5 hydrolysis of swollen cellulose Thermobifida fusca

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.74 5 9 retaines more than 50% of activity, 50ºC Thermobifida fusca
3.2.1.176 5 9 pH 5: about 45% of maximal activity, pH 9.0: about 45% of maximal activity Thermobifida fusca

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.74 Thermobifida fusca IEF gel
-
 4
3.2.1.176 Thermobifida fusca isoelectric focusing
-
 4
3.2.1.176 Thermobifida fusca calculated from sequence
-
 4.2