BRENDA - Enzyme Database

Purification and characterization of phosphoenolpyruvate carboxylase from Brassica napus (rapeseed) suspension cell cultures: implications for phosphoenolpyruvate carboxylase regulation during phosphate starvation, and the integration of glycolysis with nitrogen assimilation

Moraes, T.F.; Plaxton, W.C.; Eur. J. Biochem. 267, 4465-4476 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.31
glucose 6-phosphate
activates
Brassica napus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.31
DL-isocitrate
-
Brassica napus
4.1.1.31
L-Asp
-
Brassica napus
4.1.1.31
L-Glu
-
Brassica napus
4.1.1.31
L-malate
-
Brassica napus
4.1.1.31
quercetin
-
Brassica napus
4.1.1.31
rutin
-
Brassica napus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.31
0.08
-
phosphoenolpyruvate
pH 8.4, 24C, pH 7.3, 24C, enzyme from cells grown in presence of phosphate or in absence of phosphate, assay in presence of glycerol
Brassica napus
4.1.1.31
0.15
-
phosphoenolpyruvate
pH 7.3, 24C, pH 7.3, 24C, enzyme from cells grown in absence of phosphate, assay in presence of glycerol; pH 8.4, 24C, enzyme from cells grown in presence of phosphate, assay in absence of glycerol
Brassica napus
4.1.1.31
0.16
-
phosphoenolpyruvate
pH 8.4, 24C, enzyme from cells grown in absence of phosphate, assay in absence of glycerol
Brassica napus
4.1.1.31
0.17
-
phosphoenolpyruvate
pH 7.3, 24C, pH 7.3, 24C, enzyme from cells grown in presence of phosphate, assay in presence of glycerol
Brassica napus
4.1.1.31
0.41
-
phosphoenolpyruvate
pH 7.3, 24C, enzyme from cells grown in presence of phosphate, assay in absence of glycerol
Brassica napus
4.1.1.31
0.42
-
phosphoenolpyruvate
pH 7.3, 24C, enzyme from cells grown in absence of phosphate, assay in absence of glycerol
Brassica napus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.31
Mg2+
absolute dependence for a divalent cation, Km: 0.084 mM
Brassica napus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.31
104000
-
x * 104000, SDS-PAGE
Brassica napus
4.1.1.31
440000
-
gel filtration
Brassica napus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.31
Brassica napus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
4.1.1.31
phosphoprotein
the enzyme may exist in a dephosphorylated form in cell grown in absence of phosphate and in cells grown in presence of phosphate
Brassica napus
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.31
-
Brassica napus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.31
cell suspension culture
-
Brassica napus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.1.31
37.2
-
-
Brassica napus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.31
phosphoenolpyruvate + CO2
-
655409
Brassica napus
phosphate + oxaloacetate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.31
?
x * 104000, SDS-PAGE
Brassica napus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.31
8
9.5
-
Brassica napus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.31
7.3
8.4
activity at pH 7.3 is almost 80% of the activity at pH 8.4
Brassica napus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.31
glucose 6-phosphate
activates
Brassica napus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.31
DL-isocitrate
-
Brassica napus
4.1.1.31
L-Asp
-
Brassica napus
4.1.1.31
L-Glu
-
Brassica napus
4.1.1.31
L-malate
-
Brassica napus
4.1.1.31
quercetin
-
Brassica napus
4.1.1.31
rutin
-
Brassica napus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.31
0.08
-
phosphoenolpyruvate
pH 8.4, 24C, pH 7.3, 24C, enzyme from cells grown in presence of phosphate or in absence of phosphate, assay in presence of glycerol
Brassica napus
4.1.1.31
0.15
-
phosphoenolpyruvate
pH 7.3, 24C, pH 7.3, 24C, enzyme from cells grown in absence of phosphate, assay in presence of glycerol; pH 8.4, 24C, enzyme from cells grown in presence of phosphate, assay in absence of glycerol
Brassica napus
4.1.1.31
0.16
-
phosphoenolpyruvate
pH 8.4, 24C, enzyme from cells grown in absence of phosphate, assay in absence of glycerol
Brassica napus
4.1.1.31
0.17
-
phosphoenolpyruvate
pH 7.3, 24C, pH 7.3, 24C, enzyme from cells grown in presence of phosphate, assay in presence of glycerol
Brassica napus
4.1.1.31
0.41
-
phosphoenolpyruvate
pH 7.3, 24C, enzyme from cells grown in presence of phosphate, assay in absence of glycerol
Brassica napus
4.1.1.31
0.42
-
phosphoenolpyruvate
pH 7.3, 24C, enzyme from cells grown in absence of phosphate, assay in absence of glycerol
Brassica napus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.31
Mg2+
absolute dependence for a divalent cation, Km: 0.084 mM
Brassica napus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.31
104000
-
x * 104000, SDS-PAGE
Brassica napus
4.1.1.31
440000
-
gel filtration
Brassica napus
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
4.1.1.31
phosphoprotein
the enzyme may exist in a dephosphorylated form in cell grown in absence of phosphate and in cells grown in presence of phosphate
Brassica napus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.31
-
Brassica napus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.31
cell suspension culture
-
Brassica napus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.1.31
37.2
-
-
Brassica napus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.31
phosphoenolpyruvate + CO2
-
655409
Brassica napus
phosphate + oxaloacetate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.31
?
x * 104000, SDS-PAGE
Brassica napus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.31
8
9.5
-
Brassica napus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.31
7.3
8.4
activity at pH 7.3 is almost 80% of the activity at pH 8.4
Brassica napus