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Literature summary extracted from

  • Moraes, T.F.; Plaxton, W.C.
    Purification and characterization of phosphoenolpyruvate carboxylase from Brassica napus (rapeseed) suspension cell cultures: implications for phosphoenolpyruvate carboxylase regulation during phosphate starvation, and the integration of glycolysis with nitrogen assimilation (2000), Eur. J. Biochem., 267, 4465-4476.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 glucose 6-phosphate activates Brassica napus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 DL-isocitrate
-
 Brassica napus
4.1.1.31 L-Asp
-
 Brassica napus
4.1.1.31 L-Glu
-
 Brassica napus
4.1.1.31 L-malate
-
 Brassica napus
4.1.1.31 quercetin
-
 Brassica napus
4.1.1.31 rutin
-
 Brassica napus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 0.08
-
 phosphoenolpyruvate pH 8.4, 24°C, pH 7.3, 24°C, enzyme from cells grown in presence of phosphate or in absence of phosphate, assay in presence of glycerol Brassica napus
4.1.1.31 0.15
-
 phosphoenolpyruvate pH 7.3, 24°C, pH 7.3, 24°C, enzyme from cells grown in absence of phosphate, assay in presence of glycerol Brassica napus
4.1.1.31 0.15
-
 phosphoenolpyruvate pH 8.4, 24°C, enzyme from cells grown in presence of phosphate, assay in absence of glycerol Brassica napus
4.1.1.31 0.16
-
 phosphoenolpyruvate pH 8.4, 24°C, enzyme from cells grown in absence of phosphate, assay in absence of glycerol Brassica napus
4.1.1.31 0.17
-
 phosphoenolpyruvate pH 7.3, 24°C, pH 7.3, 24°C, enzyme from cells grown in presence of phosphate, assay in presence of glycerol Brassica napus
4.1.1.31 0.41
-
 phosphoenolpyruvate pH 7.3, 24°C, enzyme from cells grown in presence of phosphate, assay in absence of glycerol Brassica napus
4.1.1.31 0.42
-
 phosphoenolpyruvate pH 7.3, 24°C, enzyme from cells grown in absence of phosphate, assay in absence of glycerol Brassica napus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Mg2+ absolute dependence for a divalent cation, Km: 0.084 mM Brassica napus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 104000
-
 x * 104000, SDS-PAGE Brassica napus
4.1.1.31 440000
-
 gel filtration Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Brassica napus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.1.31 phosphoprotein the enzyme may exist in a dephosphorylated form in cell grown in absence of phosphate and in cells grown in presence of phosphate Brassica napus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Brassica napus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.31 cell suspension culture
-
 Brassica napus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 37.2
-
-
 Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Brassica napus phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 ? x * 104000, SDS-PAGE Brassica napus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.31 PEPC
-
 Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 8 9.5
-
 Brassica napus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.31 7.3 8.4 activity at pH 7.3 is almost 80% of the activity at pH 8.4 Brassica napus