Literature summary extracted from
Tursky, T.; Bandzuchova, E.
An endogenous activator of renal glutamic acid decarboxylase effects of adenosine triphosphate, phosphate and chloride on the activity of this enzyme (1999), Eur. J. Biochem., 262, 696-703.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
4.1.1.15 |
ADP |
2 mM, activation to about 170% of control without detergent. Activation to about 210% of control in presence of 1% Triton X-100 |
Rattus norvegicus |
|
4.1.1.15 |
AMP |
2 mM, activation to about 125% of control without detergent. Activation to about 140% of control in presence of 1% Triton X-100 |
Rattus norvegicus |
|
4.1.1.15 |
ATP |
2 mM, activation to about 150% of control in presence of 1% Triton, inhibition to 20% of control without detergent |
Rattus norvegicus |
|
4.1.1.15 |
CTP |
2 mM, activation to about 110% of control without detergent. Activation to about 200% of control in presence of 1% Triton X-100 |
Rattus norvegicus |
|
4.1.1.15 |
GTP |
2 mM, activation to about 125% of control without detergent. Activation to about 190% of control in presence of 1% Triton X-100 |
Rattus norvegicus |
|
4.1.1.15 |
UTP |
2 mM, activation to about 140% of control without detergent. Activation to about 190% of control in presence of 1% Triton X-100 |
Rattus norvegicus |
|
General Stability
EC Number |
General Stability |
Organism |
---|
4.1.1.15 |
ATP and phosphate together with chlorides stabilize renal GAD |
Rattus norvegicus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.1.1.15 |
ATP |
in presence of leupeptin in freshly prepared homogenates. Inhibition of enzyme from homogenates stored without Triton X-100 for 24 h at 4°C |
Rattus norvegicus |
|
4.1.1.15 |
EGTA |
0.5 mM, 20% inhibition |
Rattus norvegicus |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
4.1.1.15 |
membrane |
the renal enzyme differs from the brain and pancreatic enzyme by its strong binding to membranes that is not influenced by detergents |
Rattus norvegicus |
16020 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.1.1.15 |
KBr |
50 mM, enhances GAD activity in renal cortex homogenate |
Rattus norvegicus |
|
4.1.1.15 |
KCl |
50 mM, enhances GAD activity in renal cortex homogenate |
Rattus norvegicus |
|
4.1.1.15 |
LiCl |
50 mM, enhances GAD activity in renal cortex homogenate |
Rattus norvegicus |
|
4.1.1.15 |
NaBr |
50 mM, enhances GAD activity in renal cortex homogenate |
Rattus norvegicus |
|
4.1.1.15 |
NaCl |
50 mM, enhances GAD activity in renal cortex homogenate |
Rattus norvegicus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.1.15 |
Rattus norvegicus |
- |
- |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
4.1.1.15 |
kidney |
the renal enzyme differs from the brain and pancreatic enzyme by its strong binding to membranes that is not influenced by detergents |
Rattus norvegicus |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.1.15 |
L-Glu |
- |
Rattus norvegicus |
4-Aminobutanoate + CO2 |
- |
? |
|