EC Number | General Stability | Organism |
---|---|---|
1.8.98.1 | more stable in presence of CHAPS than in the presence of dodecyl beta-D-maltoside | Methanosarcina barkeri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.98.1 | 0.25 | - |
coenzyme B | pH 7.0, 37°C | Methanosarcina barkeri | |
1.8.98.1 | 0.4 | - |
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine | pH 7.0, 37°C, reaction with reduced benzyl viologen | Methanosarcina barkeri | |
1.8.98.1 | 0.8 | - |
coenzyme M | pH 7.0, 37°C | Methanosarcina barkeri |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.98.1 | membrane | - |
Methanosarcina barkeri | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.98.1 | Iron | the enzyme contains 280 nmol of non-heme iron per mg of protein | Methanosarcina barkeri |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.98.1 | 23000 | - |
x * 46000 + x * 23000, the 23000 Da subunit is cytochrome b, SDS-PAGE | Methanosarcina barkeri |
1.8.98.1 | 46000 | - |
x * 46000 + x * 23000, the 23000 Da subunit is cytochrome b, SDS-PAGE | Methanosarcina barkeri |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.98.1 | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | Methanosarcina barkeri | - |
coenzyme B + coenzyme M | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.98.1 | Methanosarcina barkeri | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.98.1 | - |
Methanosarcina barkeri |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.98.1 | culture condition:methanol-grown cell | - |
Methanosarcina barkeri | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.98.1 | 3 | 8 | - |
Methanosarcina barkeri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.98.1 | coenzyme B + coenzyme M + methylene blue | - |
Methanosarcina barkeri | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue | - |
? | |
1.8.98.1 | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2 | - |
Methanosarcina barkeri | coenzyme B + coenzyme M | - |
? | |
1.8.98.1 | N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen | - |
Methanosarcina barkeri | coenzyme B + coenzyme M + methyl viologen | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.98.1 | ? | x * 46000 + x * 23000, the 23000 Da subunit is cytochrome b, SDS-PAGE | Methanosarcina barkeri |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.98.1 | 50 | - |
- |
Methanosarcina barkeri |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.8.98.1 | 5 | 7.5 | activity with reduced benzyl viologen and N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine increases continously with decreasing pH in the range between pH 7.5 and pH 5.0 | Methanosarcina barkeri |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.98.1 | cytochrome b | the 23000 Da subunit is the cytochrome b, cytochrome b serves as electron donor for the heterodisulfide reduction | Methanosarcina barkeri | |
1.8.98.1 | FAD | the enzyme contains 3 mol of FAD per mg of protein | Methanosarcina barkeri |