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Literature summary extracted from

  • Jordaan, J.; Pletschke, B.I.; Leukes, W.D.
    Purification and partial characterization of a thermostable laccase from an unidentified basidiomycete (2004), Enzyme Microb. Technol., 34, 635-641.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 hydroxylamine competitive Basidiomycota
1.10.3.2 Sodium azide strong Basidiomycota
1.10.3.2 thioglycolic acid mixed competitive inhibition after a lag-phase Basidiomycota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0123
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.0, 25°C Basidiomycota
1.10.3.2 0.026
-
 syringic acid pH 4.5, 25°C Basidiomycota
1.10.3.2 0.251
-
 guaiacol pH 6.0, 25°C Basidiomycota
1.10.3.2 6.9
-
 3-dimethylaminobenzoic acid pH 6.0, 25°C Basidiomycota

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Basidiomycota
-
 unidentified fungal isolate
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 culture supernatant
-
 Basidiomycota
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 52
-
 pH 4.5, 25°C Basidiomycota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Basidiomycota ? + H2O
-
 ?
1.10.3.2 3-dimethylaminobenzoic acid + 3-methyl-2-benzothiazolinone hydrazone + O2 ratio of substrates 1:0.07 Basidiomycota ? + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Basidiomycota ? + H2O
-
 ?
1.10.3.2 syringic acid + O2
-
 Basidiomycota ? + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 10.1
-
 syringic acid pH 4.5, 25°C Basidiomycota
1.10.3.2 42.1
-
 guaiacol pH 6.0, 25°C Basidiomycota
1.10.3.2 368
-
 3-dimethylaminobenzoic acid pH 6.0, 25°C Basidiomycota
1.10.3.2 778
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.0, 25°C Basidiomycota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4
-
 substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Basidiomycota
1.10.3.2 4.5
-
 substrate syringic acid Basidiomycota
1.10.3.2 6
-
 substrates guaiacol, 3-dimethylaminobenzoic acid, 3-methyl-2-benzothiazolinone hydrazone Basidiomycota

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.10.3.2 0.011
-
 Sodium azide pH 4.5, 25°C Basidiomycota
1.10.3.2 0.802
-
 thioglycolic acid pH 4.5, 25°C Basidiomycota
1.10.3.2 1.01
-
 hydroxylamine pH 4.5, 25°C Basidiomycota

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 53
-
 3-dimethylaminobenzoic acid pH 6.0, 25°C Basidiomycota
1.10.3.2 168
-
 guaiacol pH 6.0, 25°C Basidiomycota
1.10.3.2 388
-
 syringic acid pH 4.5, 25°C Basidiomycota
1.10.3.2 63000
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4, 25°C Basidiomycota