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Literature summary extracted from
- Molecular cloning of the cellobiose dehydrogenase gene from Trametes versicolor and expression in Pichia pastoris (2004), Enzyme Microb. Technol., 34, 55-63.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.99.18 | expression in Pichia pastoris under the control of the AOX1 methanol promoter, without affecting its kinetic characteristics | Trametes versicolor |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 0.012 | - |
cytochrome c | pH 4.5, 25°C, wild-type enzyme | Trametes versicolor |  |
| 1.1.99.18 | 0.018 | - |
cytochrome c | pH 4.5, 25°C, recombinant enzyme | Trametes versicolor | |
| 1.1.99.18 | 0.108 | - |
cellobiose | pH 4.5, 25°C, recombinant enzyme | Trametes versicolor | |
| 1.1.99.18 | 0.128 | - |
cellobiose | pH 4.5, 25°C, wild-type enzyme | Trametes versicolor | |
| 1.1.99.18 | 0.938 | - |
lactose | pH 4.5, 25°C, recombinant enzyme | Trametes versicolor | |
| 1.1.99.18 | 0.95 | - |
lactose | pH 4.5, 25°C, wild-type enzyme | Trametes versicolor | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 98000 | - |
x * 98000, SDS-PAGE | Trametes versicolor |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.18 | Trametes versicolor | Q875J3 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | glycoprotein | the wild-type enzyme contains approximately 19% carbohydrate | Trametes versicolor |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.18 | - |
Trametes versicolor |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | additional information | - |
- |
Trametes versicolor |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | cellobiose + 2,6-dichloroindophenol | - |
Trametes versicolor | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
| 1.1.99.18 | cellobiose + 3,5-di-tert-butylbenzoquinone | - |
Trametes versicolor | cellobiono-1,5-lactone + reduced 3,5-di-tert-butylbenzoquinone | - |
? | |
| 1.1.99.18 | cellobiose + cytochrome c | - |
Trametes versicolor | cellobiono-1,5-lactone + reduced cytochrome c | - |
? | |
| 1.1.99.18 | lactose + cytochrome c | - |
Trametes versicolor | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | ? | x * 98000, SDS-PAGE | Trametes versicolor |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | Cdh | - |
Trametes versicolor |
| 1.1.99.18 | cellobiose dehydrogenase | - |
Trametes versicolor |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 50 | - |
reaction with cellobiose and cytochrome c or 3,5-di-tert-butylbenzoquinone | Trametes versicolor |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 30 | 60 | 30°C: about 70% of maximal activity, 50°C: about 80% of maximal activity, 60°C: 30% of maximal activity with wild-type enzyme, 25% of maximal activity with recombinant enzyme, reaction with cellobiose and cytochrome c or 3,5-di-tert-butylbenzoquinone | Trametes versicolor |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 40 | - |
240 min, about 20% loss of activity of wild-type enzyme, about 30% loss of activity of recombinant enzyme | Trametes versicolor |
| 1.1.99.18 | 50 | - |
60 min, about 55% loss of activity of wild-type enzyme and recombinant enzyme | Trametes versicolor |
| 1.1.99.18 | 55 | - |
20 min, about 60% loss of activity of wild-type enzyme and recombinant enzyme | Trametes versicolor |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 3.5 | - |
two activity peaks, one at pH 3.5 and another at pH 4.5-4.75, the activity at pH 3.5 is 90% of the activity at pH 4.5-7.5, reaction with cellobiose and cytochrome c | Trametes versicolor |
| 1.1.99.18 | 4 | - |
two activity peaks, one at pH 4 and another at pH 5, reaction with cellobiose and 3,5-di-tert-butylbenzoquinone | Trametes versicolor |
| 1.1.99.18 | 4.5 | 7.5 | two activity peaks, one at pH 3.5 and another at pH 4.5-4.75, the activity at pH 3.5 is 90% of the activity at pH 4.5-7.5, reaction with cellobiose and cytochrome c | Trametes versicolor |
| 1.1.99.18 | 5 | - |
two activity peaks, one at pH 4 and another at pH 5, reaction with cellobiose and 3,5-di-tert-butylbenzoquinone | Trametes versicolor |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 3.2 | 5.3 | pH 3.2: about 50% of maximal activity, pH 5.3: about 45% of maximal activity, reaction with cellobiose and cytochrome c | Trametes versicolor |
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