BRENDA - Enzyme Database show

Purification of a laccase from Marasmius quercophilus induced with ferulic acid: reactivity towards natural and xenobiotic aromatic compounds

Farnet, A.M.; Criquet, S.; Cigna, M.; Gil, G.; Ferre, E.; Enzyme Microb. Technol. 34, 549-554 (2004)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.2
azide
strong
Gymnopus quercophilus
1.10.3.2
additional information
not inhibitory: EDTA. L-cysteine
Gymnopus quercophilus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.2
0.0042
-
syringaldazine
pH 4.5
Gymnopus quercophilus
1.10.3.2
0.0113
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 4.5
Gymnopus quercophilus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.10.3.2
60000
-
x * 60000, SDS-PAGE
Gymnopus quercophilus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.10.3.2
Gymnopus quercophilus
-
induction with ferulic acid, three isozymes
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.2
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
655384
Gymnopus quercophilus
? + H2O
-
-
-
?
1.10.3.2
2,4,6-trichlorophenol + O2
-
655384
Gymnopus quercophilus
?
-
-
-
?
1.10.3.2
2,4-dichlorophenol + O2
-
655384
Gymnopus quercophilus
?
-
-
-
?
1.10.3.2
2-chlorophenol + O2
-
655384
Gymnopus quercophilus
?
-
-
-
?
1.10.3.2
syringaldazine + O2
-
655384
Gymnopus quercophilus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.10.3.2
?
x * 60000, SDS-PAGE
Gymnopus quercophilus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.10.3.2
30
-
24h, 100% residual activity, increase in activity by 80% after shift from room temperature to 30C
Gymnopus quercophilus
1.10.3.2
40
-
24h, 100% residual activity, increase in activity by 100% after shift from room temperature to 40C
Gymnopus quercophilus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.2
4
-
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Gymnopus quercophilus
1.10.3.2
4.5
-
substrate syringaldazine
Gymnopus quercophilus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.2
azide
strong
Gymnopus quercophilus
1.10.3.2
additional information
not inhibitory: EDTA. L-cysteine
Gymnopus quercophilus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.2
0.0042
-
syringaldazine
pH 4.5
Gymnopus quercophilus
1.10.3.2
0.0113
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 4.5
Gymnopus quercophilus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.10.3.2
60000
-
x * 60000, SDS-PAGE
Gymnopus quercophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.2
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
655384
Gymnopus quercophilus
? + H2O
-
-
-
?
1.10.3.2
2,4,6-trichlorophenol + O2
-
655384
Gymnopus quercophilus
?
-
-
-
?
1.10.3.2
2,4-dichlorophenol + O2
-
655384
Gymnopus quercophilus
?
-
-
-
?
1.10.3.2
2-chlorophenol + O2
-
655384
Gymnopus quercophilus
?
-
-
-
?
1.10.3.2
syringaldazine + O2
-
655384
Gymnopus quercophilus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.10.3.2
?
x * 60000, SDS-PAGE
Gymnopus quercophilus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.10.3.2
30
-
24h, 100% residual activity, increase in activity by 80% after shift from room temperature to 30C
Gymnopus quercophilus
1.10.3.2
40
-
24h, 100% residual activity, increase in activity by 100% after shift from room temperature to 40C
Gymnopus quercophilus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.2
4
-
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Gymnopus quercophilus
1.10.3.2
4.5
-
substrate syringaldazine
Gymnopus quercophilus