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Literature summary extracted from

  • Menendez, C.; Hernandez, L.; Banguela, A.; Pais, J.
    Functional production and secretion of the Gluconacetobacter diazotrophicus fructose-releasing exo-levanase (LsdB) in Pichia pastoris (2004), Enzyme Microb. Technol., 34, 446-452.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.65 synthesis commercial production of ultra-high-fructose syrups Gluconacetobacter diazotrophicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.65 expression in Pichia pastoris Gluconacetobacter diazotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.65 57000
-
 SDS-PAGE Gluconacetobacter diazotrophicus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.65 Gluconacetobacter diazotrophicus
-
-
-
3.2.1.65 Gluconacetobacter diazotrophicus SRT4
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.65 no modification
-
 Gluconacetobacter diazotrophicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.65 by immobilization-nickel affinity chromatography, centrifugation, column chromatography and dialysis Gluconacetobacter diazotrophicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.65 culture supernatant
-
 Gluconacetobacter diazotrophicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.65 58
-
 levan, pH 5.0, at 30ºC Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.65 D(+)-raffinose + H2O
-
 Gluconacetobacter diazotrophicus beta-D-fructose + alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranoside
-
 ?
3.2.1.65 D(+)-raffinose + H2O
-
 Gluconacetobacter diazotrophicus SRT4 beta-D-fructose + alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranoside
-
 ?
3.2.1.65 inulin + H2O without formation of intermediate oligofructans Gluconacetobacter diazotrophicus D-fructose + ?
-
 ?
3.2.1.65 inulin + H2O without formation of intermediate oligofructans Gluconacetobacter diazotrophicus SRT4 D-fructose + ?
-
 ?
3.2.1.65 levan + H2O without formation of intermediate oligofructans, no activity with D(+)-melezitose Gluconacetobacter diazotrophicus D-fructose + ?
-
 ?
3.2.1.65 levan + H2O without formation of intermediate oligofructans, no activity with D(+)-melezitose Gluconacetobacter diazotrophicus SRT4 D-fructose + ?
-
 ?
3.2.1.65 sucrose + H2O
-
 Gluconacetobacter diazotrophicus beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.65 sucrose + H2O
-
 Gluconacetobacter diazotrophicus SRT4 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.65 fructose-releasing exo-levanase
-
 Gluconacetobacter diazotrophicus
3.2.1.65 LsdB
-
 Gluconacetobacter diazotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.65 30
-
 levan, pH 5.0 Gluconacetobacter diazotrophicus
3.2.1.65 40 50 sucrose, pH 5.0 Gluconacetobacter diazotrophicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.65 60
-
 levan, pH 5.0, irreversibly inactived above 60ºC Gluconacetobacter diazotrophicus
3.2.1.65 60
-
 sucrose, pH 5.0, irreversibly inactived above 60ºC Gluconacetobacter diazotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.65 5
-
 levan, at 30ºC Gluconacetobacter diazotrophicus
3.2.1.65 5
-
 sucrose, at 30ºC Gluconacetobacter diazotrophicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.65 5 7 levan, at 30ºC, activity decreases at pH 4.0 and at pH values above 7.0 Gluconacetobacter diazotrophicus
3.2.1.65 5 7 sucrose, at 30ºC, activity decreases at pH 4.0 and at pH values above 7.0 Gluconacetobacter diazotrophicus