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Literature summary extracted from

  • Aguilar, G.; Morlon-Guyot, J.; Trejo-Aguilar, B.; Guyot, J.P.
    Purification and characterization of an extracellular alpha-amylase produced by Lactobacillus manihotivorans LMG 18010, an amylolytic lactic acid bacterium (2000), Enzyme Microb. Technol., 27, 406-413.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Al3+ 10 mM, 90% inhibition Lacticaseibacillus manihotivorans
3.2.1.1 Cu2+ 10 mM, 49% inhibition Lacticaseibacillus manihotivorans
3.2.1.1 Fe3+ 10 mM, 92% inhibition Lacticaseibacillus manihotivorans
3.2.1.1 Hg2+ 10 mM, 98% inhibition Lacticaseibacillus manihotivorans
3.2.1.1 Ni2+ 10 mM, 59% inhibition Lacticaseibacillus manihotivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 starch 3.44 mg/ml, 55°C Lacticaseibacillus manihotivorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular
-
 Lacticaseibacillus manihotivorans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 135000
-
 x * 135000, SDS-PAGE Lacticaseibacillus manihotivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.1 starch + H2O Lacticaseibacillus manihotivorans
-
 fragments of starch
-
 ?
3.2.1.1 starch + H2O Lacticaseibacillus manihotivorans LMG 18010
-
 fragments of starch
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Lacticaseibacillus manihotivorans
-
-
-
3.2.1.1 Lacticaseibacillus manihotivorans LMG 18010
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 DEAE-cellulose, Sephadex G-200 Lacticaseibacillus manihotivorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 additional information
-
 210 mg starch/min Lacticaseibacillus manihotivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O 90% of activity with starch Lacticaseibacillus manihotivorans fragments of amylopectin
-
 ?
3.2.1.1 amylopectin + H2O 90% of activity with starch Lacticaseibacillus manihotivorans LMG 18010 fragments of amylopectin
-
 ?
3.2.1.1 amylose + H2O 1.6times higher activity than with starch Lacticaseibacillus manihotivorans fragments of amylose
-
 ?
3.2.1.1 amylose + H2O 1.6times higher activity than with starch Lacticaseibacillus manihotivorans LMG 18010 fragments of amylose
-
 ?
3.2.1.1 dextrin + H2O 7% of activity with starch Lacticaseibacillus manihotivorans fragments of dextrin
-
 ?
3.2.1.1 dextrin + H2O 7% of activity with starch Lacticaseibacillus manihotivorans LMG 18010 fragments of dextrin
-
 ?
3.2.1.1 glycogen + H2O 7% of activity with starch Lacticaseibacillus manihotivorans fragments of glycogen
-
 ?
3.2.1.1 glycogen + H2O 7% of activity with starch Lacticaseibacillus manihotivorans LMG 18010 fragments of glycogen
-
 ?
3.2.1.1 starch + H2O
-
 Lacticaseibacillus manihotivorans fragments of starch
-
 ?
3.2.1.1 starch + H2O
-
 Lacticaseibacillus manihotivorans LMG 18010 fragments of starch
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 135000, SDS-PAGE Lacticaseibacillus manihotivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 55
-
-
 Lacticaseibacillus manihotivorans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 30 70 approx. 60% of maximal activity at 30°C and 70°C Lacticaseibacillus manihotivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 40
-
 no loss of activity after 10 min, 50% loss of activity after 100 min Lacticaseibacillus manihotivorans
3.2.1.1 50
-
 30% loss of activity after 1 h Lacticaseibacillus manihotivorans
3.2.1.1 55
-
 almost complete loss of activity after 1 h Lacticaseibacillus manihotivorans
3.2.1.1 60
-
 50% loss of activity after 10 min Lacticaseibacillus manihotivorans
3.2.1.1 80
-
 90% loss of activity after 10 min Lacticaseibacillus manihotivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6
-
-
 Lacticaseibacillus manihotivorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 3 7.5 approx. 10% of maximal activity at pH 3.0, approx. 40% of maximal activity at pH 7.0 Lacticaseibacillus manihotivorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 4 8 no loss of activity after 24 h at pH 5.0 to pH 6.0, approx. 90% loss of activity after 24 h at pH 4.0, approx. 80% loss of activity after 24 h at pH 8.0 Lacticaseibacillus manihotivorans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.1 Lacticaseibacillus manihotivorans isoelectric focusing
-
 4.2