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Literature summary extracted from

  • Georis, J.; Giannotta, F.; De Buyl, E.; Granier, B.; Frere, J.M.
    Purification and properties of three endo-beta-1,4-xylanases produced by Streptomyces sp. strain S38 which differ in their ability to enhance the bleaching of kraft pulps (2000), Enzyme Microb. Technol., 26, 178-186.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 paper production enzyme has biodelignification potential and can be used in bleaching of kraft pulps, method development, overview Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information
-
 Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular secretion of enzymes Streptomyces sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 24500
-
 x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE Streptomyces sp.
3.2.1.8 37500
-
 x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE Streptomyces sp.
3.2.1.8 38000
-
 x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 xylan + H2O Streptomyces sp. delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 ?
-
 ?
3.2.1.8 xylan + H2O Streptomyces sp. S38 delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Streptomyces sp.
-
 enzymes Xyl1, Xyl2, and Xyl3
-
3.2.1.8 Streptomyces sp. S38
-
 enzymes Xyl1, Xyl2, and Xyl3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 3 enzymes to homogeneity from culture supernatant Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.8 additional information highest enzyme production rate from culture on minimal medium at pH 7.0 and 55°C Streptomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 additional information
-
-
 Streptomyces sp.
3.2.1.8 5700
-
 purified Xyl1, substrate birchwood xylan, at 50°C Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 4-nitrophenyl arabinofuranoside + H2O Xyl2 and Xyl3 show arabinofuranosidase activity Streptomyces sp. 4-nitrophenol + arabinofuranose
-
 ?
3.2.1.8 4-nitrophenyl arabinofuranoside + H2O Xyl2 and Xyl3 show arabinofuranosidase activity Streptomyces sp. S38 4-nitrophenol + arabinofuranose
-
 ?
3.2.1.8 4-nitrophenyl cellobioside + H2O Xyl2 and Xyl3 show cellulase activity Streptomyces sp. 4-nitrophenol + cellobiose
-
 ?
3.2.1.8 4-nitrophenyl cellobioside + H2O Xyl2 and Xyl3 show cellulase activity Streptomyces sp. S38 4-nitrophenol + cellobiose
-
 ?
3.2.1.8 4-nitrophenyl xylopyranoside + H2O Xyl2 and Xyl3 show xylosidase activity Streptomyces sp. 4-nitrophenol + xylopyranose
-
 ?
3.2.1.8 additional information no activity on CM-cellulose Streptomyces sp. ?
-
 ?
3.2.1.8 additional information no activity on CM-cellulose Streptomyces sp. S38 ?
-
 ?
3.2.1.8 xylan + H2O delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 Streptomyces sp. ?
-
 ?
3.2.1.8 xylan + H2O from birchwood or oat spelt Streptomyces sp. ?
-
 ?
3.2.1.8 xylan + H2O delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 Streptomyces sp. S38 ?
-
 ?
3.2.1.8 xylan + H2O from birchwood or oat spelt Streptomyces sp. S38 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endo-beta-1,4-xylanase
-
 Streptomyces sp.
3.2.1.8 More Xyl1 belongs to family 11, while Xyl2 and Xyl3 belong to family 10 of glycohydrolases Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 Xyl1-Xyl3 are stable for 16 h Streptomyces sp.
3.2.1.8 60
-
 half-lives: Xyl1 10 min, Xyl2 30 min, Xyl3 70 min Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6 7.5 Xyl2 Streptomyces sp.
3.2.1.8 6 6.5 Xyl1 Streptomyces sp.
3.2.1.8 6 8 Xyl3 Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 3 10 Xyl1-Xyl3, no loss of activity in 7 days at 20°C Streptomyces sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Streptomyces sp. Xyl2, isoelectric focusing
-
 4.9
3.2.1.8 Streptomyces sp. Xyl3, isoelectric focusing
-
 4.9
3.2.1.8 Streptomyces sp. Xyl1, isoelectric focusing
-
 5.5