Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Biemans-Oldehinkel, E.; Poolman, B.
    On the role of the two extracytoplasmic substrate-binding domains in the ABC transporter OpuA (2003), EMBO J., 22, 5983-5993.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
7.6.2.9 additional information substrate binding domain-less mutant Lactococcus lactis
7.6.2.9 W484C mutant with 10fold increased KM for glycine betaine than the wild type enzyme Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6.2.9 0.0019
-
 glycine betaine 30ºC, pH 7.0, wild type/substrate binding domain-less mutant heterodimer Lactococcus lactis
7.6.2.9 0.0019
-
 glycine betaine 30ºC, pH 7.0, wild type/W484C heterodimer Lactococcus lactis
7.6.2.9 0.0025
-
 glycine betaine 30ºC, pH 7.0, wild type enzyme Lactococcus lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.9 membrane
-
 Lactococcus lactis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.6.2.9 Mg2+ 9 mM MgATP2- in the outside of the membranes activates OpuA Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.6.2.9 additional information
-
 the OpuA component of the wild-type enzyme runs at a lower molecular weight than that of W484C and the substrate binding domain-less mutant Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.9 Lactococcus lactis
-
-
-
7.6.2.9 Lactococcus lactis NZ9000
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.9 affinity chromatography and size-exclusion chromatography of solubilized membrane fractions Lactococcus lactis

Reaction

EC Number Reaction Comment Organism Reaction ID
7.6.2.9 ATP + H2O + quaternary amine-[quaternary amine-binding protein][side 1] = ADP + phosphate + quaternary amine[side 2] + [quaternary amine-binding protein][side 1] a single substrate binding domain per dimeric translocator complex is sufficient and minimally needed for transport. Within the complex with two substrate binding domains, the two domains interact in a cooperative manner and enhance the transport capacity Lactococcus lactis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.9 ATP + H2O + glycine betaine/out
-
 Lactococcus lactis ADP + phosphate + glycine betaine/in
-
 ?
7.6.2.9 ATP + H2O + glycine betaine/out
-
 Lactococcus lactis NZ9000 ADP + phosphate + glycine betaine/in
-
 ?

Subunits

EC Number Subunits Comment Organism
7.6.2.9 More dimeric OpuA is 220000 Da Lactococcus lactis