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Literature summary extracted from

  • Arand, M.; Hallberg, B.M.; Zou, J.; Bergfors, T.; Oesch, F.; Van Der Werf, M.J.; De Bont, J.A.M.; Jones, T.A.; Mowbray, S.L.
    Structure of Rhodococcus erythropolis limonene-1,2-epoxide hydrolase reveals a novel active site (2003), EMBO J., 22, 2583-2592.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.3.2.8 selenomethionine-substituted enzyme Rhodococcus erythropolis

Protein Variants

EC Number Protein Variants Comment Organism
3.3.2.8 D101A catalytically inactive, D101 is the acid catalyst that protonates the epoxide oxygen Rhodococcus erythropolis
3.3.2.8 D101N catalytically inactive, D101 is the acid catalyst that protonates the epoxide oxygen Rhodococcus erythropolis
3.3.2.8 N55A little residual activity Rhodococcus erythropolis
3.3.2.8 N55D catalytically inactive Rhodococcus erythropolis
3.3.2.8 N55D/D132N catalytically inactive, folding correct Rhodococcus erythropolis
3.3.2.8 R99A catalytically inactive Rhodococcus erythropolis
3.3.2.8 R99H catalytically inactive Rhodococcus erythropolis
3.3.2.8 R99K catalytically inactive Rhodococcus erythropolis
3.3.2.8 R99Q catalytically inactive Rhodococcus erythropolis
3.3.2.8 Y53F partially active Rhodococcus erythropolis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.8 hexanamide competitive Rhodococcus erythropolis
3.3.2.8 Hexylamine competitive Rhodococcus erythropolis
3.3.2.8 valpromide i.e. dipropylacetamide, competitive Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.8 1.4
-
 styrene-7,8-oxide pH 7.4, mutant N55A Rhodococcus erythropolis
3.3.2.8 1.4
-
 styrene-7,8-oxide pH 7.4, wild-type Rhodococcus erythropolis
3.3.2.8 3.7
-
 styrene-7,8-oxide pH 7.4, mutant Y53F Rhodococcus erythropolis

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.8 Rhodococcus erythropolis Q9ZAG3
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.3.2.8 1,2-epoxymenth-8-ene + H2O = menth-8-ene-1,2-diol one-step mechanism Rhodococcus erythropolis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.8 styrene-7,8-oxide + H2O
-
 Rhodococcus erythropolis styrene-7,8-diol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.3.2.8 dimer crystallization data Rhodococcus erythropolis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3.2.8 0.002
-
 styrene-7,8-oxide pH 7.4, mutant N55A Rhodococcus erythropolis
3.3.2.8 0.176
-
 styrene-7,8-oxide pH 7.4, mutant Y53F Rhodococcus erythropolis
3.3.2.8 0.47
-
 styrene-7,8-oxide pH 7.4, wild-type Rhodococcus erythropolis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.3.2.8 0.035
-
 Hexylamine pH 7.4, 1% ethanol in final assay mixture Rhodococcus erythropolis
3.3.2.8 0.1
-
 valpromide pH 7.4, 1% ethanol in final assay mixture Rhodococcus erythropolis
3.3.2.8 2
-
 hexanamide pH 7.4, 1% ethanol in final assay mixture Rhodococcus erythropolis