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Literature summary extracted from

  • Li, S.; Kelly, S.J.; Lamani, E.; Ferraroni, M.; Jedrzejas, M.J.
    Structural basis of hyaluronan degradation by Streptococcus pneumoniae hyaluronate lyase (2000), EMBO J., 19, 1228-1240.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.1 overexpression of fully functional truncated wild-type and mutant enzymes in Escherichia coli Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.1 purified recombinant truncated wid-type enzyme and selenomethionine derivative, comprising residues Ala168-Ala893, in 3.5 M ammonium sulfate, 200 mM sodium cacodylate, pH 6.0, cryoprotection in 30% xylitol, heavy atom derivative X-ray diffraction structure determination and analysis at 1.56 A resolution Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.1 H399A site-directed mutagenesis, highly reduced activity compared to wild-type enzyme Streptococcus pneumoniae
4.2.2.1 N349A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptococcus pneumoniae
4.2.2.1 N580G site-directed mutagenesis, slightly increased activity compared to wild-type enzyme Streptococcus pneumoniae
4.2.2.1 R243V site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus pneumoniae
4.2.2.1 Y408F site-directed mutagenesis, inactive mutant Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.1 Ca2+ binding modulates enzyme structure Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.1 Streptococcus pneumoniae
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.1 [hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate catalytic mechanism, substrate binding, structure modeling, residues Tyr408, Asn349, and His399 are involved in catalysis and important Streptococcus pneumoniae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.1 hyaluronan
-
Streptococcus pneumoniae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
-
?

Subunits

EC Number Subunits Comment Organism
4.2.2.1 More N-terminal alpha- and C-terminal beta-domain structure determination, determination of interface residues, overview Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
4.2.2.1 bacterial spreading factor
-
Streptococcus pneumoniae
4.2.2.1 spnHL
-
Streptococcus pneumoniae