3.2.2.3

H254A

sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme

Saccharomyces cerevisiae

3.2.2.3

construction of urh115 disruption mutant strains

Saccharomyces cerevisiae

3.2.2.3

5-fluorocytidine

noncompetitive

Saccharomyces cerevisiae

3.2.2.3

5-fluorouridine

; competitive

Saccharomyces cerevisiae

3.2.2.3

cytidine

noncompetitive

Saccharomyces cerevisiae

3.2.2.3

deoxycytidine

competitive

Saccharomyces cerevisiae

3.2.2.3

Inosine

noncompetitive

Saccharomyces cerevisiae

3.2.2.3

ribosylthymine

competitive

Saccharomyces cerevisiae

3.2.2.3

Saccharomyces cerevisiae

physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview

?

3.2.2.3

uridine + H2O

Saccharomyces cerevisiae

enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway

D-ribose + uracil

?

3.2.2.3

Saccharomyces cerevisiae

Q04179

wild-type strain MATaa and mutant derivatives, gene URH1

3.2.2.3

uridine + H2O = D-ribose + uracil

substrate binding site, active site structure, His254 is important, catalytic mechanism

Saccharomyces cerevisiae

3.2.2.3

5-fluorocytidine + H2O

655283

Saccharomyces cerevisiae

D-ribose + 5-fluorocytosine

?

3.2.2.3

5-fluorouridine + H2O

655283

Saccharomyces cerevisiae

D-ribose + 5-fluorouracil

?

3.2.2.3

cytidine + H2O

655283

Saccharomyces cerevisiae

D-ribose + cytosine

?

3.2.2.3

deoxycytidine + H2O

655283

Saccharomyces cerevisiae

D-deoxyribose + cytosine

?

3.2.2.3

physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview

655283

Saccharomyces cerevisiae

?

3.2.2.3

substrate specificity, enzyme possesses no phosphorylase activity

655283

Saccharomyces cerevisiae

?

3.2.2.3

uridine + H2O

655283

Saccharomyces cerevisiae

D-ribose + uracil

?

3.2.2.3

uridine + H2O

enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway

655283

Saccharomyces cerevisiae

D-ribose + uracil

?

3.2.2.3

Urh1p

Saccharomyces cerevisiae

3.2.2.3

uridine/cytidine hydrolase

Saccharomyces cerevisiae

3.2.2.3

37

assay at

Saccharomyces cerevisiae

3.2.2.3

7.5

assay at

Saccharomyces cerevisiae

3.2.2.3

0.6

5-fluorouridine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

2

cytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

2

ribosylthymine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

3

5-fluorocytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

3

deoxycytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

3

Inosine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

H254A

sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme

Saccharomyces cerevisiae

3.2.2.3

construction of urh115 disruption mutant strains

Saccharomyces cerevisiae

3.2.2.3

5-fluorocytidine

noncompetitive

Saccharomyces cerevisiae

3.2.2.3

5-fluorouridine

Saccharomyces cerevisiae

3.2.2.3

5-fluorouridine

competitive

Saccharomyces cerevisiae

3.2.2.3

cytidine

noncompetitive

Saccharomyces cerevisiae

3.2.2.3

deoxycytidine

competitive

Saccharomyces cerevisiae

3.2.2.3

Inosine

noncompetitive

Saccharomyces cerevisiae

3.2.2.3

ribosylthymine

competitive

Saccharomyces cerevisiae

3.2.2.3

0.6

5-fluorouridine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

2

cytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

2

ribosylthymine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

3

5-fluorocytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

3

deoxycytidine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

3

Inosine

pH 7.5, 37°C, wild-type enzyme, versus uridine

Saccharomyces cerevisiae

3.2.2.3

Saccharomyces cerevisiae

physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview

?

3.2.2.3

uridine + H2O

Saccharomyces cerevisiae

enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway

D-ribose + uracil

?

3.2.2.3

5-fluorocytidine + H2O

655283

Saccharomyces cerevisiae

D-ribose + 5-fluorocytosine

?

3.2.2.3

5-fluorouridine + H2O

655283

Saccharomyces cerevisiae

D-ribose + 5-fluorouracil

?

3.2.2.3

cytidine + H2O

655283

Saccharomyces cerevisiae

D-ribose + cytosine

?

3.2.2.3

deoxycytidine + H2O

655283

Saccharomyces cerevisiae

D-deoxyribose + cytosine

?

3.2.2.3

physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview

655283

Saccharomyces cerevisiae

?

3.2.2.3

substrate specificity, enzyme possesses no phosphorylase activity

655283

Saccharomyces cerevisiae

?

3.2.2.3

uridine + H2O

655283

Saccharomyces cerevisiae

D-ribose + uracil

?

3.2.2.3

uridine + H2O

enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway

655283

Saccharomyces cerevisiae

D-ribose + uracil

?

3.2.2.3

37

assay at

Saccharomyces cerevisiae

3.2.2.3

7.5

assay at

Saccharomyces cerevisiae