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Literature summary extracted from

  • Kurtz, J.E.; Exinger, F.; Erbs, P.; Jund, R.
    The URH1 uridine ribohydrolase of Saccharomyces cerevisiae (2002), Curr. Genet., 41, 132-141.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.3 H254A sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme Saccharomyces cerevisiae
3.2.2.3 additional information construction of urh115 disruption mutant strains Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.3 5-fluorocytidine noncompetitive Saccharomyces cerevisiae
3.2.2.3 5-fluorouridine competitive Saccharomyces cerevisiae
3.2.2.3 cytidine noncompetitive Saccharomyces cerevisiae
3.2.2.3 deoxycytidine competitive Saccharomyces cerevisiae
3.2.2.3 Inosine noncompetitive Saccharomyces cerevisiae
3.2.2.3 ribosylthymine competitive Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.3 additional information Saccharomyces cerevisiae physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview ?
-
 ?
3.2.2.3 uridine + H2O Saccharomyces cerevisiae enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway D-ribose + uracil
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.3 Saccharomyces cerevisiae Q04179 wild-type strain MATaa and mutant derivatives, gene URH1
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.3 uridine + H2O = D-ribose + uracil substrate binding site, active site structure, His254 is important, catalytic mechanism Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.3 5-fluorocytidine + H2O
-
 Saccharomyces cerevisiae D-ribose + 5-fluorocytosine
-
 ?
3.2.2.3 5-fluorouridine + H2O
-
 Saccharomyces cerevisiae D-ribose + 5-fluorouracil
-
 ?
3.2.2.3 cytidine + H2O
-
 Saccharomyces cerevisiae D-ribose + cytosine
-
 ?
3.2.2.3 deoxycytidine + H2O
-
 Saccharomyces cerevisiae D-deoxyribose + cytosine
-
 ?
3.2.2.3 additional information physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview Saccharomyces cerevisiae ?
-
 ?
3.2.2.3 additional information substrate specificity, enzyme possesses no phosphorylase activity Saccharomyces cerevisiae ?
-
 ?
3.2.2.3 uridine + H2O
-
 Saccharomyces cerevisiae D-ribose + uracil
-
 ?
3.2.2.3 uridine + H2O enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway Saccharomyces cerevisiae D-ribose + uracil
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.3 Urh1p
-
 Saccharomyces cerevisiae
3.2.2.3 uridine/cytidine hydrolase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.3 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.3 7.5
-
 assay at Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.2.3 0.6
-
 5-fluorouridine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae
3.2.2.3 2
-
 cytidine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae
3.2.2.3 2
-
 ribosylthymine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae
3.2.2.3 3
-
 Inosine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae
3.2.2.3 3
-
 deoxycytidine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae
3.2.2.3 3
-
 5-fluorocytidine pH 7.5, 37°C, wild-type enzyme, versus uridine Saccharomyces cerevisiae