Data extracted from this reference:
Engineering
3.2.2.3
H254A
sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme
Saccharomyces cerevisiae
3.2.2.3
additional information
construction of urh115 disruption mutant strains
Saccharomyces cerevisiae
Inhibitors
3.2.2.3
5-fluorocytidine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
5-fluorouridine
; competitive
Saccharomyces cerevisiae
3.2.2.3
cytidine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
deoxycytidine
competitive
Saccharomyces cerevisiae
3.2.2.3
Inosine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
ribosylthymine
competitive
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
3.2.2.3
additional information
Saccharomyces cerevisiae
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
?
3.2.2.3
uridine + H2O
Saccharomyces cerevisiae
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
D-ribose + uracil
?
Organism
3.2.2.3
Saccharomyces cerevisiae
Q04179
wild-type strain MATaa and mutant derivatives, gene URH1
Reaction
3.2.2.3
uridine + H2O = D-ribose + uracil
substrate binding site, active site structure, His254 is important, catalytic mechanism
Saccharomyces cerevisiae
Substrates and Products (Substrate)
3.2.2.3
5-fluorocytidine + H2O
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorocytosine
?
3.2.2.3
5-fluorouridine + H2O
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorouracil
?
3.2.2.3
cytidine + H2O
655283
Saccharomyces cerevisiae
D-ribose + cytosine
?
3.2.2.3
deoxycytidine + H2O
655283
Saccharomyces cerevisiae
D-deoxyribose + cytosine
?
3.2.2.3
additional information
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
655283
Saccharomyces cerevisiae
?
3.2.2.3
additional information
substrate specificity, enzyme possesses no phosphorylase activity
655283
Saccharomyces cerevisiae
?
3.2.2.3
uridine + H2O
655283
Saccharomyces cerevisiae
D-ribose + uracil
?
3.2.2.3
uridine + H2O
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
655283
Saccharomyces cerevisiae
D-ribose + uracil
?
Synonyms
3.2.2.3
Urh1p
Saccharomyces cerevisiae
3.2.2.3
uridine/cytidine hydrolase
Saccharomyces cerevisiae
Temperature Optimum [°C]
3.2.2.3
37
assay at
Saccharomyces cerevisiae
pH Optimum
3.2.2.3
7.5
assay at
Saccharomyces cerevisiae
Ki Value [mM]
3.2.2.3
0.6
5-fluorouridine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
2
cytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
2
ribosylthymine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
5-fluorocytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
deoxycytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
Inosine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
Engineering (protein specific)
3.2.2.3
H254A
sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme
Saccharomyces cerevisiae
3.2.2.3
additional information
construction of urh115 disruption mutant strains
Saccharomyces cerevisiae
Inhibitors (protein specific)
3.2.2.3
5-fluorocytidine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
5-fluorouridine
Saccharomyces cerevisiae
3.2.2.3
5-fluorouridine
competitive
Saccharomyces cerevisiae
3.2.2.3
cytidine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
deoxycytidine
competitive
Saccharomyces cerevisiae
3.2.2.3
Inosine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
ribosylthymine
competitive
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
3.2.2.3
0.6
5-fluorouridine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
2
cytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
2
ribosylthymine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
5-fluorocytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
deoxycytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
Inosine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
3.2.2.3
additional information
Saccharomyces cerevisiae
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
?
3.2.2.3
uridine + H2O
Saccharomyces cerevisiae
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
D-ribose + uracil
?
Substrates and Products (Substrate) (protein specific)
3.2.2.3
5-fluorocytidine + H2O
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorocytosine
?
3.2.2.3
5-fluorouridine + H2O
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorouracil
?
3.2.2.3
cytidine + H2O
655283
Saccharomyces cerevisiae
D-ribose + cytosine
?
3.2.2.3
deoxycytidine + H2O
655283
Saccharomyces cerevisiae
D-deoxyribose + cytosine
?
3.2.2.3
additional information
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
655283
Saccharomyces cerevisiae
?
3.2.2.3
additional information
substrate specificity, enzyme possesses no phosphorylase activity
655283
Saccharomyces cerevisiae
?
3.2.2.3
uridine + H2O
655283
Saccharomyces cerevisiae
D-ribose + uracil
?
3.2.2.3
uridine + H2O
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
655283
Saccharomyces cerevisiae
D-ribose + uracil
?
Temperature Optimum [°C] (protein specific)
3.2.2.3
37
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
3.2.2.3
7.5
assay at
Saccharomyces cerevisiae