show The URH1 uridine ribohydrolase of Saccharomyces cerevisiae
Kurtz, J.E.; Exinger, F.; Erbs, P.; Jund, R.; Curr. Genet. 41, 132-141 (2002)
Data extracted from this reference:
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.2.2.3
H254A
sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme
Saccharomyces cerevisiae
3.2.2.3
additional information
construction of urh115 disruption mutant strains
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.3
5-fluorocytidine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
5-fluorouridine
; competitive
Saccharomyces cerevisiae
3.2.2.3
cytidine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
deoxycytidine
competitive
Saccharomyces cerevisiae
3.2.2.3
Inosine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
ribosylthymine
competitive
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.3
additional information
Saccharomyces cerevisiae
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
?
-
-
-
3.2.2.3
uridine + H2O
Saccharomyces cerevisiae
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
D-ribose + uracil
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.2.3
Saccharomyces cerevisiae
Q04179
wild-type strain MATaa and mutant derivatives, gene URH1
-
Reaction
EC Number
Reaction
Commentary
Organism
3.2.2.3
uridine + H2O = D-ribose + uracil
substrate binding site, active site structure, His254 is important, catalytic mechanism
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.3
5-fluorocytidine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorocytosine
-
-
-
?
3.2.2.3
5-fluorouridine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorouracil
-
-
-
?
3.2.2.3
cytidine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + cytosine
-
-
-
?
3.2.2.3
deoxycytidine + H2O
-
655283
Saccharomyces cerevisiae
D-deoxyribose + cytosine
-
-
-
?
3.2.2.3
additional information
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
655283
Saccharomyces cerevisiae
?
-
-
-
-
3.2.2.3
additional information
substrate specificity, enzyme possesses no phosphorylase activity
655283
Saccharomyces cerevisiae
?
-
-
-
-
3.2.2.3
uridine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + uracil
-
-
-
?
3.2.2.3
uridine + H2O
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
655283
Saccharomyces cerevisiae
D-ribose + uracil
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.2.3
37
-
assay at
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.3
7.5
-
assay at
Saccharomyces cerevisiae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.2.3
0.6
-
5-fluorouridine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
2
-
cytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
2
-
ribosylthymine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
-
5-fluorocytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
-
deoxycytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
-
Inosine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.2.2.3
H254A
sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme
Saccharomyces cerevisiae
3.2.2.3
additional information
construction of urh115 disruption mutant strains
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.3
5-fluorocytidine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
5-fluorouridine
-
Saccharomyces cerevisiae
3.2.2.3
5-fluorouridine
competitive
Saccharomyces cerevisiae
3.2.2.3
cytidine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
deoxycytidine
competitive
Saccharomyces cerevisiae
3.2.2.3
Inosine
noncompetitive
Saccharomyces cerevisiae
3.2.2.3
ribosylthymine
competitive
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.2.3
0.6
-
5-fluorouridine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
2
-
cytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
2
-
ribosylthymine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
-
5-fluorocytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
-
deoxycytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
3.2.2.3
3
-
Inosine
pH 7.5, 37°C, wild-type enzyme, versus uridine
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.3
additional information
Saccharomyces cerevisiae
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
?
-
-
-
3.2.2.3
uridine + H2O
Saccharomyces cerevisiae
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
D-ribose + uracil
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.3
5-fluorocytidine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorocytosine
-
-
-
?
3.2.2.3
5-fluorouridine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + 5-fluorouracil
-
-
-
?
3.2.2.3
cytidine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + cytosine
-
-
-
?
3.2.2.3
deoxycytidine + H2O
-
655283
Saccharomyces cerevisiae
D-deoxyribose + cytosine
-
-
-
?
3.2.2.3
additional information
physiological function, investigation of metabolic fluxes in several mutants impaired in the pyrimidine salvage pathway, regulation, overview
655283
Saccharomyces cerevisiae
?
-
-
-
-
3.2.2.3
additional information
substrate specificity, enzyme possesses no phosphorylase activity
655283
Saccharomyces cerevisiae
?
-
-
-
-
3.2.2.3
uridine + H2O
-
655283
Saccharomyces cerevisiae
D-ribose + uracil
-
-
-
?
3.2.2.3
uridine + H2O
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
655283
Saccharomyces cerevisiae
D-ribose + uracil
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.2.3
37
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.3
7.5
-
assay at
Saccharomyces cerevisiae