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Literature summary extracted from

  • Iwaki-Egawa, S.; Watanabe, Y.
    Characterization and purification of adenosine deaminase 1 from human and chicken liver (2002), Comp. Biochem. Physiol. B, 133, 173-182.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.4 2'-deoxycoformycin 0.0015 nM, 50% inhibition Gallus gallus
3.5.4.4 2'-deoxycoformycin 0.0004 nM, 50% inhibition Homo sapiens
3.5.4.4 erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride 0.4 nM, 50% inhibition Gallus gallus
3.5.4.4 erythro-9-(2-hydroxy-3-nonyl)-adenine hydrochloride 0.45 nM, 50% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.4 0.000023
-
 adenosine
-
 Gallus gallus
3.5.4.4 0.000046
-
 adenosine
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.4 42000
-
 a second band of 300000 Da is detected for the complex of adenosine deaminase 1 with dipeptidyl peptidase IV, non-denaturing PAGE Homo sapiens
3.5.4.4 42000
-
 1 * 42000, enzyme exists as monomer, not in complex with dipeptidyl peptidase IV, SDS-PAGE Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.4 Gallus gallus
-
-
-
3.5.4.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.4
-
 Gallus gallus
3.5.4.4
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.4 liver
-
 Gallus gallus
-
3.5.4.4 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.4 20
-
-
 Gallus gallus
3.5.4.4 28.49
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.4 2',3'-deoxyadenosine + H2O 1.7% of the activity with adenosine Gallus gallus 2',3'-deoxyinosine + NH3
-
 ?
3.5.4.4 2'-deoxyadenosine + H2O 79.2% of the activity with adenosine Gallus gallus 2'-deoxyinosine + NH3
-
 ?
3.5.4.4 2'-deoxyadenosine + H2O 82.8% of the activity with adenosine Homo sapiens 2'-deoxyinosine + NH3
-
 ?
3.5.4.4 3'-deoxyadenosine + H2O 2.4% of the activity with adenosine Gallus gallus 3'-deoxyinosine + NH3
-
 ?
3.5.4.4 adenosine + H2O
-
 Gallus gallus inosine + NH3
-
 ?
3.5.4.4 adenosine + H2O
-
 Homo sapiens inosine + NH3
-
 ?
3.5.4.4 additional information activity with 3'-deoxyadenosine, 2',3'-deoxyadenosine and 2'-O-methyladenosine is less than 0.5% of the activity with adenine Homo sapiens ?
-
 ?
3.5.4.4 additional information no activity with 2'-O-methyladenosine Gallus gallus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.4 monomer 1 * 42000, enzyme exists as monomer, not in complex with dipeptidyl peptidase IV, SDS-PAGE Gallus gallus
3.5.4.4 monomer 1 * 42000, in human liver the adenosine deaminase 1 mainly exists as a complex with dipeptidyl peptidase IV. The molar ratio of adenosine deaminase 1 to dipeptidyl peptidase IV appears to be 2:1, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.5.4.4 ADA1
-
 Gallus gallus
3.5.4.4 ADA1
-
 Homo sapiens
3.5.4.4 adenosine deaminase 1
-
 Gallus gallus
3.5.4.4 adenosine deaminase 1
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.4 7.5 8
-
 Gallus gallus
3.5.4.4 7.5 8
-
 Homo sapiens