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Literature summary extracted from

  • Iriyama, N.; Takeuchi, N.; Shiraishi, T.; Izumi, K.; Sawada, M.T.; Takahashi, N.; Furuhata, K.; Ogura, H.; Uda, Y.
    Enzymatic properties of sialidase from the ovary of the starfish, Asterina pectinifera (2000), Comp. Biochem. Physiol. B, 126, 561-569.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.18 2,3-dehydro-2-deoxysialic acid 70.2% inhibition at 0.1 mM Patiria pectinifera
3.2.1.18 4-aminophenyl thioglucoside derivatives of sialic acid
-
 Patiria pectinifera
3.2.1.18 chondroitin sulfate A
-
 Patiria pectinifera
3.2.1.18 D-glucosamine 6-sulfate slight inhibition at 10 mM Patiria pectinifera
3.2.1.18 D-glucose 6-sulfate slight inhibition at 10 mM Patiria pectinifera
3.2.1.18 Dextran sulfate
-
 Patiria pectinifera
3.2.1.18 heparin
-
 Patiria pectinifera
3.2.1.18 methyl thioglucoside derivatives of sialic acid
-
 Patiria pectinifera
3.2.1.18 additional information no inhibition by 5-amino sialic acid and sialic acid methyl ester Patiria pectinifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.18 55000
-
 6 * 55000, SDS-PAGE Patiria pectinifera
3.2.1.18 360000
-
 gel filtration Patiria pectinifera

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 Patiria pectinifera
-
 starfish
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18 12287fold, by ammonium precipitation, ultrafiltration, reverse phase, gel filtration and ConA-affinity chromatography, and preparative PAGE Patiria pectinifera

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.18 colominic acid + H2O = sialic acid + lactose enzyme does not act on 4-O-acetylated sialic acids Patiria pectinifera
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.18 ovary
-
 Patiria pectinifera
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.18 17.2
-
 purified enzyme Patiria pectinifera

Storage Stability

EC Number Storage Stability Organism
3.2.1.18 -20°C, purified enzyme, sodium acetate, pH 4.5, stable for 1 year Patiria pectinifera
3.2.1.18 4°C, purified enzyme, sodium acetate, pH 4.5, stable for 2 months Patiria pectinifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
 Patiria pectinifera 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
 ?
3.2.1.18 alpha(2-3)-sialyllactose + H2O high activity Patiria pectinifera sialic acid + lactose
-
 ?
3.2.1.18 alpha(2-6)-sialyllactose + H2O
-
 Patiria pectinifera sialic acid + lactose
-
 ?
3.2.1.18 colominic acid + H2O
-
 Patiria pectinifera sialic acid + lactose
-
 ?
3.2.1.18 fetuin + H2O
-
 Patiria pectinifera sialic acid + ?
-
 ?
3.2.1.18 ganglioside GD1a + H2O
-
 Patiria pectinifera sialic acid + ?
-
 ?
3.2.1.18 ganglioside GD1b + H2O
-
 Patiria pectinifera sialic acid + ?
-
 ?
3.2.1.18 ganglioside GM3 + H2O best substrate Patiria pectinifera sialic acid + ?
-
 ?
3.2.1.18 additional information ganglioside GM1 and bovine serum albumin are poor substrates, no activity with 4-methylumbelliferyl-alpha-3-deoxy-D-glycero-D-galacto-2-nonulosonic acid Patiria pectinifera ?
-
 ?
3.2.1.18 transferrin + H2O
-
 Patiria pectinifera sialic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.18 hexamer 6 * 55000, SDS-PAGE Patiria pectinifera

Synonyms

EC Number Synonyms Comment Organism
3.2.1.18 sialidase
-
 Patiria pectinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.18 3 4
-
 Patiria pectinifera