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Literature summary extracted from

  • Kohring, G.W.; Wiehr, P.; Jeworski, M.; Giffhorn, F.
    Stereoselective oxidation of aliphatic diols and reduction of hydroxy-ketones with galactitol dehydrogenase from Rhodobacter sphaeroides D (2003), Commun. Agric. Appl. Biol. Sci., 68, 309-312.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.406 synthesis preparation of optically pure aliphatic diols by enzymic oxidation of one enantiomer or stereospecific reduction of keto-alcohols or diketones Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.406 0.5
-
(S)-1,2,6-hexanetriol pH 6.5, 35°C Cereibacter sphaeroides
1.1.1.406 1.4
-
2,3-Hexanedione pH 6.5, 35°C Cereibacter sphaeroides
1.1.1.406 1.4
-
(S)-1,2-hexanediol pH 6.5, 35°C Cereibacter sphaeroides
1.1.1.406 4.2
-
3,4-hexanedione pH 6.5, 35°C Cereibacter sphaeroides
1.1.1.406 26
-
1-hydroxy-2-butanone pH 6.5, 35°C Cereibacter sphaeroides
1.1.1.406 49
-
(S)-1,2-butanediol pH 6.5, 35°C Cereibacter sphaeroides
1.1.1.406 196
-
(S)-1,2-Propanediol pH 6.5, 35°C Cereibacter sphaeroides
1.1.1.406 202
-
Hydroxyacetone pH 6.5, 35°C Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.406 26657
-
x * 26657, MALDI-TOF-MS Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.406 Cereibacter sphaeroides
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.406 (S)-1,2,6-hexanetriol + NAD+
-
Cereibacter sphaeroides 1,6-dihydroxy-2-hexanone + NADH
-
r
1.1.1.406 (S)-1,2-butanediol + NAD+
-
Cereibacter sphaeroides 1-hydroxy-2-butanone + NADH
-
r
1.1.1.406 (S)-1,2-hexanediol + NAD+
-
Cereibacter sphaeroides 1-hydroxy-2-hexanone + NADH
-
r
1.1.1.406 (S)-1,2-propanediol + NAD+
-
Cereibacter sphaeroides hydroxyacetone + NADH
-
r
1.1.1.406 1-hydroxy-2-butanone + NADH
-
Cereibacter sphaeroides (S)-1,2-butanediol + NAD+
-
r
1.1.1.406 2,3-hexandione + NADH
-
Cereibacter sphaeroides (2R,3S)-hexanediol + NAD+
-
r
1.1.1.406 3,4-hexanedione + NADH
-
Cereibacter sphaeroides ? + NAD+ reaction product is a complex polymer r
1.1.1.406 hydroxyacetone + NADH
-
Cereibacter sphaeroides (S)-1,2-propanediol + NAD+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.406 ? x * 26657, MALDI-TOF-MS Cereibacter sphaeroides