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Literature summary extracted from

  • Fong, W.P.; Cheng, C.H.K.; Tang, W.K.
    Purification and characterization of two distinct aldehyde oxidizing enzymes from the liver of black seabream (2003), Chem. -Biol. Interact., 143-144, 159-167.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.007
-
 propanal pH 9.5 Acanthopagrus schlegelii
1.2.1.3 0.007
-
 octanal pH 9.5 Acanthopagrus schlegelii
1.2.1.3 0.009
-
 acetaldehyde pH 9.5 Acanthopagrus schlegelii
1.2.1.3 0.4
-
 NAD+ pH 9.5, reaction with acetaldehyde Acanthopagrus schlegelii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 56000
-
 x * 56000, SDS-PAGE Acanthopagrus schlegelii

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Acanthopagrus schlegelii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3
-
 Acanthopagrus schlegelii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 liver
-
 Acanthopagrus schlegelii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.3 8.5
-
-
 Acanthopagrus schlegelii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Acanthopagrus schlegelii acetate + NADH + H+
-
 ?
1.2.1.3 additional information no activity with benzaldehyde Acanthopagrus schlegelii ?
-
 ?
1.2.1.3 octanal + NAD+ + H2O
-
 Acanthopagrus schlegelii octanoate + NADH
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Acanthopagrus schlegelii propanoate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 ? x * 56000, SDS-PAGE Acanthopagrus schlegelii

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 ALDH-2
-
 Acanthopagrus schlegelii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.3 20
-
 pH 7.5, 10 min, stable in presence of 2 mM NAD+ Acanthopagrus schlegelii
1.2.1.3 30
-
 pH 7.5, 10 min, about 25% loss of activity in presence of 2 mM NAD+ Acanthopagrus schlegelii
1.2.1.3 40
-
 pH 7.5, 10 min, about 75% loss of activity Acanthopagrus schlegelii
1.2.1.3 50
-
 pH 7.5, 10 min, complete loss of activity Acanthopagrus schlegelii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 8.5 9
-
 Acanthopagrus schlegelii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.3 7.5 10 pH 7.5: about 40% of maximal activity, pH 10.0: about 60% of maximal activity, reaction with acetaldehyde Acanthopagrus schlegelii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.3 6 8 10 min, stable in presence of 2 mM NAD+ Acanthopagrus schlegelii