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Literature summary extracted from

  • Ishikura, S.; Isaji, T.; Usami, N.; Nakagawa, J.; El-Kabbani, O.; Hara, A.
    Identification of amino acid residues involved in substrate recognition of L-xylulose reductase by site-directed mutagenesis (2003), Chem. Biol. Interact., 143-144, 543-550.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.10 expression of wild-type and mutant enzymes in Escherichia coli Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.10 H146L site-directed mutagenesis, altered activity Rattus norvegicus
1.1.1.10 K153M site-directed mutagenesis, active site mutant, complete loss of activity Rattus norvegicus
1.1.1.10 L143F site-directed mutagenesis, altered activity Rattus norvegicus
1.1.1.10 N190V site-directed mutagenesis, altered activity Rattus norvegicus
1.1.1.10 N190V/W191S site-directed mutagenesis, almost complete loss of L-xylulose reductase activity Rattus norvegicus
1.1.1.10 N190V/W191S/Q137M/L143F/H146L site-directed mutagenesis, almost complete loss of L-xylulose reductase activity, mutant shows high 3-ketosteroid reductase activity Rattus norvegicus
1.1.1.10 Q137M site-directed mutagenesis, altered activity, stable against cold inactivation Rattus norvegicus
1.1.1.10 Q137M/F241L site-directed mutagenesis, altered activity, sensitive to cold inactivation like the wild-type enzyme Rattus norvegicus
1.1.1.10 Q137M/L143F site-directed mutagenesis, increased Km for L-xylulose compared to the wild-type Rattus norvegicus
1.1.1.10 Q137M/L143F/H146L site-directed mutagenesis, almost complete loss of L-xylulose reductase activity, mutant shows 3-ketosteroid reductase activity Rattus norvegicus
1.1.1.10 S136A site-directed mutagenesis, active site mutant, complete loss of activity Rattus norvegicus
1.1.1.10 W191F site-directed mutagenesis, altered activity Rattus norvegicus
1.1.1.10 W191S site-directed mutagenesis, altered activity Rattus norvegicus
1.1.1.10 Y149F site-directed mutagenesis, active site mutant, complete loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.10 n-butyric acid specific, binds to the enzyme-NADP+ complex, the mutant enzymes show altered sensitivity to inhibition, overview Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.10 0.00067
-
 NADP+ pH 7.0, 25°C, mutant S136A Rattus norvegicus
1.1.1.10 0.0013
-
 NADP+ pH 7.0, 25°C, mutant Y149F Rattus norvegicus
1.1.1.10 0.002
-
 NADPH pH 7.0, 25°C, mutant Y149F Rattus norvegicus
1.1.1.10 0.003
-
 NADP+ pH 7.0, 25°C, wild-type enzyme Rattus norvegicus
1.1.1.10 0.0046
-
 NADPH pH 7.0, 25°C, mutant S136A Rattus norvegicus
1.1.1.10 0.005
-
 1,4-dibromo-2,3-butanedione pH 7.0, 25°C, wild-type enzyme Rattus norvegicus
1.1.1.10 0.007
-
 NADPH pH 7.0, 25°C, wild-type enzyme Rattus norvegicus
1.1.1.10 0.0099
-
 L-xylitol pH 7.0, 25°C, mutant Y149F Rattus norvegicus
1.1.1.10 0.011
-
 L-xylitol pH 7.0, 25°C, wild-type enzyme Rattus norvegicus
1.1.1.10 0.013
-
 L-xylitol pH 7.0, 25°C, mutant S136A Rattus norvegicus
1.1.1.10 0.05
-
 1,4-dibromo-2,3-butanedione pH 7.0, 25°C, mutant S136A Rattus norvegicus
1.1.1.10 0.053
-
 NADPH pH 7.0, 25°C, mutant K153M Rattus norvegicus
1.1.1.10 0.085
-
 L-xylitol pH 7.0, 25°C, mutant K153M Rattus norvegicus
1.1.1.10 0.092
-
 L-xylulose pH 7.0, 25°C, mutant H146L Rattus norvegicus
1.1.1.10 0.1
-
 1,4-dibromo-2,3-butanedione pH 7.0, 25°C, mutant K153M Rattus norvegicus
1.1.1.10 0.117
-
 NADP+ pH 7.0, 25°C, mutant K153M Rattus norvegicus
1.1.1.10 0.139
-
 1,4-dibromo-2,3-butanedione pH 7.0, 25°C, mutant Y149F Rattus norvegicus
1.1.1.10 0.14
-
 L-xylulose pH 7.0, 25°C, wild-type enzyme and mutant L143F Rattus norvegicus
1.1.1.10 0.31
-
 L-xylulose pH 7.0, 25°C, mutant N190V Rattus norvegicus
1.1.1.10 0.33
-
 L-xylulose pH 7.0, 25°C, mutant Q137M Rattus norvegicus
1.1.1.10 0.78
-
 diacetyl pH 7.0, 25°C, wild-type enzyme Rattus norvegicus
1.1.1.10 1.2
-
 diacetyl pH 7.0, 25°C, mutant H146L Rattus norvegicus
1.1.1.10 2.6
-
 diacetyl pH 7.0, 25°C, mutant N190V Rattus norvegicus
1.1.1.10 2.7
-
 diacetyl pH 7.0, 25°C, mutant N190V/W191S/Q137M/L143F/H146L Rattus norvegicus
1.1.1.10 3
-
 diacetyl pH 7.0, 25°C, mutant Q137M Rattus norvegicus
1.1.1.10 3.6
-
 L-xylulose pH 7.0, 25°C, mutant Q137M/L143F Rattus norvegicus
1.1.1.10 5.2
-
 diacetyl pH 7.0, 25°C, mutant W191F Rattus norvegicus
1.1.1.10 6.2
-
 diacetyl pH 7.0, 25°C, mutant L143F Rattus norvegicus
1.1.1.10 7.2
-
 L-xylulose pH 7.0, 25°C, mutant W191F Rattus norvegicus
1.1.1.10 9.6
-
 diacetyl pH 7.0, 25°C, mutant Q137M/L143F/H146L Rattus norvegicus
1.1.1.10 11
-
 diacetyl pH 7.0, 25°C, mutant Q137M/L143F Rattus norvegicus
1.1.1.10 16
-
 diacetyl pH 7.0, 25°C, mutant N190V/W191S Rattus norvegicus
1.1.1.10 42
-
 diacetyl pH 7.0, 25°C, mutant W191S Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.10 membrane
-
 Rattus norvegicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.10 26500
-
 x * 26500 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.10 diacetyl + NAD(P)H Rattus norvegicus detoxification of alpha-dicarbonyl compounds acetoin + NAD(P)+
-
 r
1.1.1.10 L-xylulose + NADPH + H+ Rattus norvegicus part of the uronate cycle, involved in osmoregulation in the kidney L-xylitol + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.10 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.10 xylitol + NADP+ = L-xylulose + NADPH + H+ enzyme with dual function showing L-xylulose reductase and dicarbonyl reductase activities, it is probably identical with sperm 34 kDa protein P34H and diacetyl reductase, EC 1.1.1.5, the amino acid residues Ser136, Tyr149, and Lys153 form the catalytic triad Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.10 kidney brush border Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.10 1,4-dibromo-2,3-butanedione + NADPH dicarbonyl reductase activity Rattus norvegicus ? + NADP+
-
 r
1.1.1.10 diacetyl + NAD(P)H detoxification of alpha-dicarbonyl compounds Rattus norvegicus acetoin + NAD(P)+
-
 r
1.1.1.10 diacetyl + NAD(P)H dicarbonyl reductase activity Rattus norvegicus acetoin + NAD(P)+
-
 r
1.1.1.10 L-xylulose + NADPH + H+ part of the uronate cycle, involved in osmoregulation in the kidney Rattus norvegicus L-xylitol + NADP+
-
 r
1.1.1.10 L-xylulose + NADPH + H+ L-xylulose reductase activity Rattus norvegicus L-xylitol + NADP+
-
 r
1.1.1.10 additional information the size and hydrophobicity of the amino acid residues involved in substrate recognition, i.e. Q137, L143, H146, N190, and W191, is important, mutants N190V, N190V/W191S, Q137M/L143F/H146L, and N190V/W191S/Q137M/L143F/H146L show reductive activity with 4-nitroacetophenone, 5beta-androstane-3,17-dione, 5beta-androstan-17beta-ol-3-one, and 5beta-androstane-3alpha,17beta-diol, overview Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.10 ? x * 26500 Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.10 More enzyme belongs to the short-chain dehydrogenase/reductase family Rattus norvegicus
1.1.1.10 XR
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.10 25
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.10 0.0005
-
 NADPH pH 7.0, 25°C, mutant K153M Rattus norvegicus
1.1.1.10 0.0006
-
 NADPH pH 7.0, 25°C, mutant S136A Rattus norvegicus
1.1.1.10 0.0009
-
 NADPH pH 7.0, 25°C, mutant Y149F Rattus norvegicus
1.1.1.10 9.5
-
 NADP+ pH 7.0, 25°C, wild-type enzyme Rattus norvegicus
1.1.1.10 25
-
 NADPH pH 7.0, 25°C, wild-type enzyme Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.10 7
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.10 NADP+
-
 Rattus norvegicus
1.1.1.10 NADPH
-
 Rattus norvegicus