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Literature summary extracted from

  • Nidetzky, B.; Helmer, H.; Klimacek, M.; Lunzer, R.; Mayer, G.
    Characterization of recombinant xylitol dehydrogenase from Galactocandida mastotermitis expressed in Escherichia coli (2003), Chem. Biol. Interact., 143-144, 533-542.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.9 additional information no significant content of Mg2+ Candida sp. HA 167
1.1.1.9 Zn2+ 0.9 molecules per subunit Candida sp. HA 167

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.9 38000
-
 4 * 38000, SDS-PAGE Candida sp. HA 167
1.1.1.9 160000
-
 gel filtration Candida sp. HA 167

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Candida sp. HA 167
-
 expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.9 recombinant enzyme Candida sp. HA 167

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 D-fructose + NADH + H+
-
 Candida sp. HA 167 D-sorbitol + NAD+
-
 ?
1.1.1.9 D-sorbitol + NAD+
-
 Candida sp. HA 167 D-fructose + NADH + H+
-
 r
1.1.1.9 D-xylitol + NAD+
-
 Candida sp. HA 167 D-xylulose + NADH + H+
-
 r
1.1.1.9 D-xylulose + NADH + H+ specific for transferring the 4-pro-R hydrogen of NADH Candida sp. HA 167 D-xylitol + NAD+
-
 r
1.1.1.9 additional information specific for transferring the 4-pro-R hydrogen of NADH Candida sp. HA 167 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.9 homotetramer 4 * 38000, SDS-PAGE Candida sp. HA 167

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.9 114
-
 D-sorbitol 25°C, pH 7.5, Tris-HCl Candida sp. HA 167
1.1.1.9 143
-
 xylitol 25°C, pH 7.5, Tris-HCl Candida sp. HA 167
1.1.1.9 170
-
 xylitol 25°C, pH 7.5, potassium phosphate Candida sp. HA 167
1.1.1.9 920
-
 D-fructose 25°C, pH 7.5, Tris-HCl Candida sp. HA 167
1.1.1.9 1500
-
 D-xylulose 25°C, pH 7.5, Tris-HCl Candida sp. HA 167
1.1.1.9 1800
-
 D-xylulose 25°C, pH 7.5, potassium phosphate Candida sp. HA 167

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+
-
 Candida sp. HA 167
1.1.1.9 NADH
-
 Candida sp. HA 167

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.9 Candida sp. HA 167 isoelectric focusing
-
 6