EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.50 | F118A | site-directed mutagenesis, mutation of a substrate binding residue, altered steroid recognition and kinetics compared to the wild-type enzyme | Rattus norvegicus |
1.1.1.50 | F129A | site-directed mutagenesis, mutation of a substrate binding residue, altered steroid recognition and kinetics compared to the wild-type enzyme, highly reduced activity | Rattus norvegicus |
1.1.1.50 | L54A | site-directed mutagenesis, mutation of a substrate binding residue, altered steroid recognition and kinetics compared to the wild-type enzyme | Rattus norvegicus |
1.1.1.50 | N306A | site-directed mutagenesis, mutation of a substrate binding residue, altered steroid recognition and kinetics compared to the wild-type enzyme | Rattus norvegicus |
1.1.1.50 | T226A | site-directed mutagenesis, mutation of a substrate binding residue, steroid recognition and kinetics are similar to the wild-type enzyme | Rattus norvegicus |
1.1.1.50 | T24A | site-directed mutagenesis, mutation of a substrate binding residue, steroid recognition and kinetics are similar to the wild-type enzyme | Rattus norvegicus |
1.1.1.50 | W227A | site-directed mutagenesis, mutation of a substrate binding residue, altered steroid recognition and kinetics compared to the wild-type enzyme, highly reduced activity and binding of progesterone | Rattus norvegicus |
1.1.1.50 | Y310A | site-directed mutagenesis, mutation of a substrate binding residue, altered steroid recognition and kinetics compared to the wild-type enzyme | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.50 | additional information | - |
additional information | kinetics | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | additional information | Rattus norvegicus | enzyme plays a pivotal role in the inactivation of circulating steroid hormones | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.50 | Rattus norvegicus | - |
isozyme AKR1C9 | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.50 | a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | ordered bi bi kinetic mechanism, catalytic residues are Y55 and H117 | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.50 | liver | isozyme AKR1C9 | Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | additional information | enzyme plays a pivotal role in the inactivation of circulating steroid hormones | Rattus norvegicus | ? | - |
? | |
1.1.1.50 | additional information | wild-type enzyme recognizes only the 3-keto function of steroids, while the substrate binding residue-mutant enzymes show a broader substrate specificity and broader reaction spectra, overview, structure-function study on the basis of the crystal structure, modeling | Rattus norvegicus | ? | - |
? | |
1.1.1.50 | progesterone + NADPH | - |
Rattus norvegicus | ? | - |
? | |
1.1.1.50 | testosterone + NADPH + H+ | substrate binding residues are T24, L54, Y55, H117, F118, F129, T226, W227, N306, and Y310 | Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.50 | More | structure-function study on the basis of the crystal structure, modeling | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.50 | 3alpha-HSD | - |
Rattus norvegicus |
1.1.1.50 | 3alpha-hydroxysteroid dehydrogenase | - |
Rattus norvegicus |
1.1.1.50 | AKR1C9 | - |
Rattus norvegicus |
1.1.1.50 | More | enzyme belongs to the aldo-keto reductase AKR superfamily | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 37 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 7 | - |
assay at | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.50 | NADPH | - |
Rattus norvegicus |