EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.50 | medicine | enzyme is a potential drug target | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.50 | expression of isozymes AKR1C1-4 in Escherichia coli | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.50 | isozyme AKR1C2 in ternary complex with NADP+ and ursodeoxycholate, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement | Homo sapiens |
1.1.1.50 | isozyme AKR1C9 in ternary complex with NADP+ and ursodeoxycholate, X-ray diffraction structure determination and analysis at 2.8 A resolution | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.50 | additional information | - |
additional information | cofactor binding kinetics | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | 5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | Homo sapiens | isozyme AKR1C4 | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
1.1.1.50 | 5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | Rattus norvegicus | isozyme AKR1C9 | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
1.1.1.50 | additional information | Rattus norvegicus | enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | ? | - |
? | |
1.1.1.50 | additional information | Homo sapiens | physiological roles of the isozymes AKR1C1-4, overview, enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.50 | Homo sapiens | - |
4 isozymes AKR1C1-4 | - |
1.1.1.50 | Rattus norvegicus | - |
isozyme AKR1C9 | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.50 | a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | steroid binding pocket structure of isozyme AKR1C2 | Homo sapiens | |
1.1.1.50 | a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | steroid binding pocket structure of isozyme AKR1C9 | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.50 | liver | isozyme AKR1C4 | Homo sapiens | - |
1.1.1.50 | prostate | isozyme AKR1C4 | Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | additional information | - |
- |
Homo sapiens |
1.1.1.50 | additional information | - |
- |
Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | 5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | isozyme AKR1C4 | Homo sapiens | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
1.1.1.50 | 5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | isozyme AKR1C9 | Rattus norvegicus | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
1.1.1.50 | 5alpha-dihydroprogesterone + 2 NADPH + 2 H+ | preferred substrate for isozyme AKR1C4, while isozymes AKR1C1-3 show only low activity | Homo sapiens | pregnan-3alpha,20beta-diol + 2 NADP+ | - |
r | |
1.1.1.50 | additional information | enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | Rattus norvegicus | ? | - |
? | |
1.1.1.50 | additional information | physiological roles of the isozymes AKR1C1-4, overview, enzyme regulates the occupancy and trans-activation of steroid hormone receptors by interconverting potent hormones with their cognate inactive metabolites | Homo sapiens | ? | - |
? | |
1.1.1.50 | additional information | isozyme is positional- and stereospecific for the 3alpha-hydroxy steroid dehydrogenase reaction | Rattus norvegicus | ? | - |
? | |
1.1.1.50 | additional information | isozymes display 3alpha-, 17beta-, and 20alpha-hydroxysteroid dehydrogenase activities to varying degrees, isozyme AKR1C4 shows the highest 3alpha-hydroxysteroid dehydrogenase activity of all isozymes, overview | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.50 | More | isozyme AKR1C2 shows an (alphabeta)8-barrel structure | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.50 | 3alpha-hydroxysteroid dehydrogenase | - |
Homo sapiens |
1.1.1.50 | 3alpha-hydroxysteroid dehydrogenase | - |
Rattus norvegicus |
1.1.1.50 | AKR1C1-4 | - |
Homo sapiens |
1.1.1.50 | AKR1C9 | - |
Rattus norvegicus |
1.1.1.50 | HSD | - |
Homo sapiens |
1.1.1.50 | HSD | - |
Rattus norvegicus |
1.1.1.50 | More | enzyme belongs to the aldo-keto reductase superfamily | Homo sapiens |
1.1.1.50 | More | enzyme belongs to the aldo-keto reductase superfamily | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.50 | 0.02 | - |
5alpha-dihydrotestosterone | isozyme AKR1C2 | Homo sapiens | |
1.1.1.50 | 1 | - |
5alpha-dihydrotestosterone | isozyme AKR1C9 | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.50 | NADP+ | binding and release mechanism | Homo sapiens | |
1.1.1.50 | NADP+ | binding and release mechanism | Rattus norvegicus | |
1.1.1.50 | NADPH | binding and release mechanism | Homo sapiens | |
1.1.1.50 | NADPH | binding and release mechanism | Rattus norvegicus |