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Literature summary extracted from

  • Luonteri, E.; Laine, C.; Uusitalo, S.; Teleman, A.; Siika-aho, M.; Tenkanen, M.
    Purification and characterization of Aspergillus beta-D-galactanases acting on beta-1,4- and beta-1,3/6-linked arabinogalactans (2003), Carbohydr. Polym., 53, 155-168.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.89 38000
-
gel filtration Aspergillus aculeatus
3.2.1.89 38000
-
SDS-PAGE, gel filtration Aspergillus aculeatus
3.2.1.164 17000
-
gel filtration, the enzyme shows unusual behaviour in gel filtration Aspergillus niger
3.2.1.164 58000
-
1 * 58000, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.89 additional information Aspergillus aculeatus activity with galactans from different sources, overview ?
-
?
3.2.1.164 additional information Aspergillus niger activity with galactans from different sources, overview ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.89 Aspergillus aculeatus
-
-
-
3.2.1.89 Aspergillus aculeatus
-
from a commercial product Pectinex Ultra SP-L
-
3.2.1.164 Aspergillus niger
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.89 additional information no glycoprotein Aspergillus aculeatus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.89 from a commercial product Pectinex Ultra SP-L Aspergillus aculeatus
3.2.1.89 native enzyme from commercial pectinase preparation by anion and cation exchange chromatography, ultrafiltration, and gel filtration Aspergillus aculeatus
3.2.1.164 native enzyme from commercial pectinase preparation by anion and cation exchange chromatography, ultrafiltration, and gel filtration Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.89 commercial preparation commercial pectinase preparation Aspergillus aculeatus
-
3.2.1.164 commercial preparation commercial pectinase preparation Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.89 3059
-
purified enzyme Aspergillus aculeatus
3.2.1.89 3060
-
-
Aspergillus aculeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.89 beta-1,4-galactan + H2O enzyme produces a range of galacto-oligolaccharides, main products are galactose and galactobiose Aspergillus aculeatus ?
-
?
3.2.1.89 galactan I + H2O isolated from black liquor, enzyme produces a range of galacto-oligolaccharides, main products are galactose and galactobiose Aspergillus aculeatus ?
-
?
3.2.1.89 additional information enzyme liberates galactose from a lignin-carbohydrate complex isolated from softwood kraft pulp, no activity towards beta-1,3-linked galactan Aspergillus aculeatus ?
-
?
3.2.1.89 additional information activity with galactans from different sources, overview Aspergillus aculeatus ?
-
?
3.2.1.89 additional information the enzyme is highly active and specific for beta-galactans hydrolyzing beta-1,4-galactosidic linkages, substrate specificity, the enzyme acts on beta-1,4-linked galactan, producing a range of galacto-oligosaccharides, and on beta-1,4- and beta-1,3/6-linked arabinogalactans, the enzyme liberates galactose from a lignin–carbohydrate complex isolated from softwood kraft pulp, no activity towards beta-1,3-liked galactan, overview Aspergillus aculeatus ?
-
?
3.2.1.164 additional information activity with galactans from different sources, overview Aspergillus niger ?
-
?
3.2.1.164 additional information the beta-1,6-galactanase liberates galactose and 1,6-galactobiose from acid-treated larchwood and Norway spruce arabinogalactans Aspergillus niger ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.89 monomer 1 * 38000, SDS-PAGE Aspergillus aculeatus
3.2.1.164 monomer 1 * 58000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.89 beta-D-galactanase
-
Aspergillus aculeatus
3.2.1.89 endo-beta-1,4-D-galactanase
-
Aspergillus aculeatus
3.2.1.164 beta-D-galactanase
-
Aspergillus niger
3.2.1.164 endo-beta-1,6-D-galactanase
-
Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.89 50
-
assay at Aspergillus aculeatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.89 40
-
up to, purified enzyme, pH 4.5, 24 h, 80% remaining activity Aspergillus aculeatus
3.2.1.89 45
-
purified enzyme, pH 4.5, 24 h, 47% remaining activity Aspergillus aculeatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.89 4.5
-
-
Aspergillus aculeatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.89 3.4 5 enzyme acts efficiently between pH 3.4 and pH 5 Aspergillus aculeatus
3.2.1.89 3.4 5 high activity within this range Aspergillus aculeatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.89 3.2 7.5 enzyme retains at least 80% of its activity between pH 4.4 and pH 7 at 40°C after 24 h, but loses 50% of its activity below pH 3.2 and above pH 7.5 Aspergillus aculeatus
3.2.1.89 3.2 7.5 purified enzyme, 24 h, 40°C, 80% remaining activity at pH 4.4 and pH 7.0, less than 50% remaining activity below pH 3.2 and above pH 7.5 Aspergillus aculeatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.89 Aspergillus aculeatus below, isoelectric focusing
-
4
3.2.1.89 Aspergillus aculeatus pI below 4, chromatofocusing
-
4