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Literature summary extracted from

  • Panagiotou, G.; Topakas, E.; Economou, L.; Kekos, D.; Macris, B.J.; Christakopoulos, P.
    Induction, purification, and characterization of two extracellular alpha-L-arabinofuranosidases from Fusarium oxysporum (2003), Can. J. Microbiol., 49, 639-644.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.28
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 45°C, isozyme ABF2 Fusarium oxysporum
3.2.1.55 0.39
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 45°C, isozyme ABF1 Fusarium oxysporum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 extracellular
-
 Fusarium oxysporum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 180000
-
 isozyme ABF2 Fusarium oxysporum
3.2.1.55 200000
-
 isozyme ABF1 Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Fusarium oxysporum
-
 2 isozymes ABF1 and ABF2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 ABF1, 7.9fold, and ABF2, 20.4fold, to homogeneity Fusarium oxysporum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.55 additional information effect of carbon sources in the medium on enzyme production of the culture, overview Fusarium oxysporum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 3.88
-
 purified isozyme ABF1 Fusarium oxysporum
3.2.1.55 10
-
 purified isozyme ABF2 Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Fusarium oxysporum 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 arabinan + H2O from sugar beet, not from wheat Fusarium oxysporum alpha-L-arabinofuranose
-
 ?
3.2.1.55 arabinoxylan + H2O soluble or insoluble Fusarium oxysporum alpha-L-arabinofuranose + ?
-
 ?
3.2.1.55 additional information no activity with substrates containing ferulic acid ester linked to C5 and C2 linkages of 4-nitrophenyl alpha-L-arabinofuranoside Fusarium oxysporum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 trimer
-
 Fusarium oxysporum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 ABF
-
 Fusarium oxysporum
3.2.1.55 alpha-L-arabinofuranosidase
-
 Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 50 55 isozyme ABF2 Fusarium oxysporum
3.2.1.55 55 60 isozyme ABF1 Fusarium oxysporum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 50
-
 1 h, complete loss of activity of isozyme ABF1, 37% remaining activity of isozyme ABF2 Fusarium oxysporum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.55 Fusarium oxysporum isozyme ABF1
-
 6
3.2.1.55 Fusarium oxysporum isozyme ABF2
-
 7.1