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Literature summary extracted from

  • Martel, M.B.; Herve du Penhoat, C.; Letoublon, R.; Fevre, M.
    Purification and characterization of a glucoamylase secreted by the plant pathogen Scierotinia sclerotiorum (2002), Can. J. Microbiol., 48, 212-218.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 starch 1 mg/ml, 50°C, pH 4.0 Sclerotinia sclerotiorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.3 Ca2+ 1 mM, 50% increase in activity Sclerotinia sclerotiorum
3.2.1.3 Mn2+ 1 mM, 20% increase in activity Sclerotinia sclerotiorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 72000
-
 x * 72000, SDS-PAGE Sclerotinia sclerotiorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 amylose + H2O Sclerotinia sclerotiorum 13% of activity with starch beta-D-glucose + ?
-
 ?
3.2.1.3 glycogen + 3 H2O Sclerotinia sclerotiorum 150% of activity with starch 4 beta-D-glucose
-
 ?
3.2.1.3 starch + H2O Sclerotinia sclerotiorum
-
 beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Sclerotinia sclerotiorum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein
-
 Sclerotinia sclerotiorum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 ammonium sulfate, G-25 Sephadex, Mono Q, Econo-Pac S, TSK 2000 Gel Sclerotinia sclerotiorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 4-nitrophenyl alpha-D-glucopyranoside + H2O 12% of activity with starch Sclerotinia sclerotiorum 4-nitrophenol + D-glucose
-
 ?
3.2.1.3 amylose + H2O 13% of activity with starch Sclerotinia sclerotiorum beta-D-glucose + ?
-
 ?
3.2.1.3 glycogen + 3 H2O 150% of activity with starch Sclerotinia sclerotiorum 4 beta-D-glucose
-
 ?
3.2.1.3 starch + H2O
-
 Sclerotinia sclerotiorum beta-D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 72000, SDS-PAGE Sclerotinia sclerotiorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 50
-
-
 Sclerotinia sclerotiorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 3.5 4
-
 Sclerotinia sclerotiorum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.3 Sclerotinia sclerotiorum isoelectric focusing, probably 4 to 5 isoforms with pIs from 4.8 to 5.4
-
 6