EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.5.2 | biotechnology | enzyme has a great potential for application as glucose sensor constituent | Acinetobacter calcoaceticus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.5.2 | expression of wild-type isozyme PQQGDH-B and mutant enzymes in Escherichia coli | Acinetobacter calcoaceticus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.5.2 | N340F/Y418F | site-directed mutagenesis, mutation of residues at the dimer interface, 2fold increased thermal stability at 55°C and unaltered catalytic efficiency compared to the wild-type enzyme | Acinetobacter calcoaceticus |
1.1.5.2 | N340F/Y418I | site-directed mutagenesis, mutation of residues at the dimer interface, 2fold increased thermal stability at 55°C and unaltered catalytic efficiency compared to the wild-type enzyme | Acinetobacter calcoaceticus |
1.1.5.2 | T416V/T417V | site-directed mutagenesis, mutation of resides of the hydrophobic region, 2fold increased thermal stability at 55°C and unaltered catalytic efficiency compared to the wild-type enzyme | Acinetobacter calcoaceticus |
1.1.99.35 | D340F/Y418F | increase in temperature stability | Acinetobacter calcoaceticus |
1.1.99.35 | D340F/Y418I | increase in temperature stability | Acinetobacter calcoaceticus |
1.1.99.35 | T416V/T417V | increase in temperature stability | Acinetobacter calcoaceticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.2 | additional information | - |
additional information | predicted binding energy of wild-type and mutant enzymes | Acinetobacter calcoaceticus | |
1.1.5.2 | 16 | - |
D-glucose | recombinant mutant T416V/T417V, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
1.1.5.2 | 20 | - |
D-glucose | recombinant wild-type enzyme and mutants N340F/Y418F and N340F/Y418I, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 16 | - |
D-glucose | mutant T416V/T417V, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 20 | - |
D-glucose | wild-type, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 20 | - |
D-glucose | mutant D340F/Y418F, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
1.1.99.35 | 20 | - |
D-glucose | mutant D340F/Y418I, pH 7.0, 25°C | Acinetobacter calcoaceticus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.5.2 | soluble | isozyme PQQGDH-B | Acinetobacter calcoaceticus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.2 | Ca2+ | - |
Acinetobacter calcoaceticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.2 | D-glucose + ubiquinone | Acinetobacter calcoaceticus | - |
D-glucono-1,5-lactone + ubiquinol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.2 | Acinetobacter calcoaceticus | P13650 | isozyme PQQGDH-B | - |
1.1.99.35 | Acinetobacter calcoaceticus | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.5.2 | 2500 | - |
mutant N340F/Y418I | Acinetobacter calcoaceticus |
1.1.5.2 | 2800 | - |
mutant T416V/T417V | Acinetobacter calcoaceticus |
1.1.5.2 | 3030 | - |
wild-type isozyme PQQGDH-B | Acinetobacter calcoaceticus |
1.1.5.2 | 3100 | - |
mutant N340F/Y418F | Acinetobacter calcoaceticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.2 | D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
1.1.5.2 | D-glucose + ubiquinone | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
1.1.99.35 | D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.5.2 | dimer | structure analysis, 6-blade beta-propeller protein with each blade consisting of a 4-stranded anti-parallel beta-sheet | Acinetobacter calcoaceticus |
1.1.5.2 | More | the monomeric enzyme is not active, 3D models of wild-type and mutant enzymes | Acinetobacter calcoaceticus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.2 | PQQ glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
1.1.5.2 | PQQGDH-B | - |
Acinetobacter calcoaceticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.2 | 25 | - |
assay at | Acinetobacter calcoaceticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.2 | 55 | - |
wild-type isozyme PQQGDH-B: half-life 9.5 min | Acinetobacter calcoaceticus |
1.1.99.35 | 53.9 | - |
wild-type, 50% loss of initial activity within in 10 min | Acinetobacter calcoaceticus |
1.1.99.35 | 55 | - |
half-life of wild-type, 9.5 min, of mutant D340F/Y418F 14.9 min, of mutant D340F/Y418I 15.5 min, of mutant T416V/T417V 14.8 min, respectively | Acinetobacter calcoaceticus |
1.1.99.35 | 56.5 | - |
mutant T416V/T417V, 50% loss of initial activity within in 10 min | Acinetobacter calcoaceticus |
1.1.99.35 | 57.5 | - |
mutant D340F/Y418I, 50% loss of initial activity within in 10 min | Acinetobacter calcoaceticus |
1.1.99.35 | 57.7 | - |
mutant D340F/Y418F, 50% loss of initial activity within in 10 min | Acinetobacter calcoaceticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.5.2 | 7 | - |
assay at | Acinetobacter calcoaceticus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.2 | pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus |