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Literature summary extracted from

  • Pollegioni, L.; Caldinelli, L.; Molla, G.; Sacchi, S.; Pilone, M.S.
    Catalytic properties of D-amino acid oxidase in cephalosporin C bioconversion: a comparison between proteins from different sources (2004), Biotechnol. Prog., 20, 467-473.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 7-(5-oxoadipoamido)cephalosporanic acid product inhibition Rhodotorula toruloides
1.4.3.3 7-(5-oxoadipoamido)cephalosporanic acid product inhibition Sus scrofa
1.4.3.3 7-(5-oxoadipoamido)cephalosporanic acid product inhibition Trigonopsis variabilis
1.4.3.3 glutaryl-7-aminocephalosporanic acid noncompetitive Rhodotorula toruloides
1.4.3.3 glutaryl-7-aminocephalosporanic acid
-
 Sus scrofa
1.4.3.3 glutaryl-7-aminocephalosporanic acid noncompetitive Trigonopsis variabilis
1.4.3.3 H2O2 product inhibition, competitive versus cephalosporin Rhodotorula toruloides
1.4.3.3 H2O2 product inhibition, noncompetitive versus cephalosporin Sus scrofa
1.4.3.3 H2O2 product inhibition, competitive versus cephalosporin Trigonopsis variabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 1
-
 D-Ala pH 8.5, 25°C Rhodotorula toruloides
1.4.3.3 1.7
-
 D-Ala pH 8.5, 25°C Sus scrofa
1.4.3.3 2.3
-
 cephalosporin C pH 8.5, 25°C Sus scrofa
1.4.3.3 2.4
-
 cephalosporin C pH 8.5, 25°C Trigonopsis variabilis
1.4.3.3 4
-
 cephalosporin C pH 8.5, 25°C Rhodotorula toruloides
1.4.3.3 7
-
 D-Ala pH 8.5, 25°C Trigonopsis variabilis

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Rhodotorula toruloides
-
-
-
1.4.3.3 Sus scrofa
-
-
-
1.4.3.3 Trigonopsis variabilis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.3 kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 cephalosporin C + H2O + O2
-
 Sus scrofa 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
1.4.3.3 cephalosporin C + H2O + O2
-
 Rhodotorula toruloides 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
1.4.3.3 cephalosporin C + H2O + O2
-
 Trigonopsis variabilis 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-Ala + H2O + O2
-
 Sus scrofa pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Ala + H2O + O2
-
 Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Ala + H2O + O2
-
 Trigonopsis variabilis pyruvate + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 DAAO
-
 Sus scrofa
1.4.3.3 DAAO
-
 Rhodotorula toruloides
1.4.3.3 DAAO
-
 Trigonopsis variabilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.3 35
-
 30 min, about 30% loss of activity Sus scrofa
1.4.3.3 35
-
 30 min, about 30% loss of activity Rhodotorula toruloides
1.4.3.3 40
-
 30 min, stable Trigonopsis variabilis
1.4.3.3 40
-
 30 min, about 40% loss of activity Sus scrofa
1.4.3.3 40
-
 30 min, about 95% loss of activity Rhodotorula toruloides
1.4.3.3 45
-
 30 min, about 50% loss of activity Sus scrofa
1.4.3.3 45
-
 30 min, about 15% loss of activity Trigonopsis variabilis
1.4.3.3 50
-
 30 min, about 75% loss of activity Sus scrofa
1.4.3.3 55
-
 30 min, complete loss of activity Sus scrofa
1.4.3.3 55
-
 30 min, about 55% loss of activity Trigonopsis variabilis
1.4.3.3 60
-
 30 min, about 80% loss of activity Trigonopsis variabilis
1.4.3.3 65
-
 30 min, complete loss of activity Trigonopsis variabilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 39
-
 cephalosporin C pH 8.5, 25°C Sus scrofa
1.4.3.3 440
-
 D-Ala pH 8.5, 25°C Sus scrofa
1.4.3.3 2740
-
 D-Ala pH 8.5, 25°C Trigonopsis variabilis
1.4.3.3 4300
-
 cephalosporin C pH 8.5, 25°C Trigonopsis variabilis
1.4.3.3 4400
-
 cephalosporin C pH 8.5, 25°C Rhodotorula toruloides
1.4.3.3 4880
-
 D-Ala pH 8.5, 25°C Rhodotorula toruloides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.3 7.5
-
-
 Trigonopsis variabilis
1.4.3.3 9
-
-
 Rhodotorula toruloides
1.4.3.3 9 10
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.3 6.5 10 pH 6.5: about 55% of maximal activity, pH 10.0: about 50% of maximal activity Trigonopsis variabilis
1.4.3.3 6.5 10 pH 6.5: about 55% of maximal activity, pH 10.0: about 80% of maximal activity Rhodotorula toruloides
1.4.3.3 8.4 10 pH 8.4: about 60% of maximal activity, pH 9.0-10.0: maximum Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.3 9.5
-
 maximal stability Sus scrofa
1.4.3.3 9.5
-
 maximal stability Rhodotorula toruloides
1.4.3.3 9.5
-
 maximal stability Trigonopsis variabilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.3 0.16 1.4 glutaryl-7-aminocephalosporanic acid pH 8.5, 25°C Sus scrofa
1.4.3.3 3.9
-
 H2O2 pH 8.5, 25°C Sus scrofa
1.4.3.3 8.1 25.3 glutaryl-7-aminocephalosporanic acid pH 8.5, 25°C Trigonopsis variabilis
1.4.3.3 10 14.1 7-(5-oxoadipoamido)cephalosporanic acid pH 8.5, 25°C Sus scrofa
1.4.3.3 11.8 26.5 glutaryl-7-aminocephalosporanic acid pH 8.5, 25°C Rhodotorula toruloides
1.4.3.3 23.6
-
 7-(5-oxoadipoamido)cephalosporanic acid pH 8.5, 25°C Rhodotorula toruloides
1.4.3.3 27.2
-
 7-(5-oxoadipoamido)cephalosporanic acid pH 8.5, 25°C Trigonopsis variabilis
1.4.3.3 33.1
-
 H2O2 pH 8.5, 25°C Rhodotorula toruloides
1.4.3.3 36
-
 H2O2 pH 8.5, 25°C Trigonopsis variabilis