EC Number | General Stability | Organism |
---|---|---|
1.4.3.3 | multisubunit immobilization on highly activated glyoxyl agarose improves stability of the enzyme 1500fold, at a protein concentration of 0.0067 mg/ml. | Rhodotorula toruloides |
1.4.3.3 | multisubunit immobilization on highly activated glyoxyl agarose marginally improves stability of the enzyme, 15-20fold, at a protein concentration of 0.0067 mg/ml. Dissociation of FAD is not prevented by immobilization | Trigonopsis variabilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.3 | Rhodotorula toruloides | - |
- |
- |
1.4.3.3 | Trigonopsis variabilis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.3 | cephalosporin C + H2O + O2 | - |
Rhodotorula toruloides | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
1.4.3.3 | cephalosporin C + H2O + O2 | - |
Trigonopsis variabilis | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.3 | RgDAAO | - |
Rhodotorula toruloides |
1.4.3.3 | TvDAAO | - |
Trigonopsis variabilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.3 | 40 | - |
50 h, pH 7.5, about 45% loss of activity of enzyme after multisubunit immobilization on highly activated glyoxyl agarose, protein concentration 0.0067 mg/ml | Rhodotorula toruloides |
1.4.3.3 | 40 | - |
50 h, pH 7.5, about 45% loss of activity of enzyme after multisubunit immobilization on highly activated glyoxyl agarose, protein concentration 0.2 mg/ml | Trigonopsis variabilis |
1.4.3.3 | 42 | - |
50 h, pH 7.5, about 60% loss of activity of enzyme after multisubunit immobilization on highly activated glyoxyl agarose, protein concentration 0.067 mg/ml and 50 mM FAD, 95% loss of activity without FAD | Trigonopsis variabilis |