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Literature summary extracted from

  • Thompson, V.S.; Schaller, K.D.; Apel, W.A.
    Purification and characterization of a novel thermo-alkali-stable catalase from Thermus brockianus (2003), Biotechnol. Prog., 19, 1292-1299.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.11.1.6 industry enzyme has potential for application in industrial bleaching processes to remove residual hydrogen peroxide from process streams Thermus brockianus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 3-Amino-1,2,4-triazole strong inhibition Thermus brockianus
1.11.1.6 CN- strong inhibition Thermus brockianus
1.11.1.6 additional information no inhibition by H2O2 up to 450 mM Thermus brockianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 35.5
-
 H2O2 pH 8.0, 70°C Thermus brockianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 42500
-
 4 * 42500, SDS-PAGE Thermus brockianus
1.11.1.6 178000
-
 gel filtration Thermus brockianus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Thermus brockianus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 3-step procedure, 65fold Thermus brockianus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 5320
-
 purified enzyme Thermus brockianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2
-
 Thermus brockianus O2 + H2O
-
 ?
1.11.1.6 additional information enzyme is easiliy reduced by dithionite, no peroxidase activity with o-dianisidine and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Thermus brockianus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 tetramer 4 * 42500, SDS-PAGE Thermus brockianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 90
-
-
 Thermus brockianus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.6 30 94 inactive at 20°C Thermus brockianus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 80
-
 pH 8.0, half-life: 330 h Thermus brockianus
1.11.1.6 90
-
 pH 8.0, half-life: 3 h Thermus brockianus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.6 360000
-
 H2O2 pH 8.0, 70°C Thermus brockianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 8
-
-
 Thermus brockianus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.6 6 10
-
 Thermus brockianus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.6 9
-
 70°C, half-life: 510 h Thermus brockianus
1.11.1.6 10
-
 70°C, half-life: 360 h Thermus brockianus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.6 heme c 8 molecules per teramer Thermus brockianus
1.11.1.6 additional information no protoheme IX Thermus brockianus