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Literature summary extracted from

  • Tochihara, T.; Sasaki, K.; Araki, O.; Morimoto, N.; Watanabe, K.; Hatada, Y.; Ito, S.; Ito, H.; Matsui, H.
    Site-directed mutagenesis establishes aspartic acids-227 and -342 as essential for enzyme activity in an isomalto-dextranase from Arthrobacter globiformis (2004), Biotechnol. Lett., 26, 659-664.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.94
-
 Arthrobacter globiformis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.94 D107A activity similar to wild-type enzyme Arthrobacter globiformis
3.2.1.94 D163A activity similar to wild-type enzyme Arthrobacter globiformis
3.2.1.94 D227A no activity of dextran hydrolysis Arthrobacter globiformis
3.2.1.94 D227E decreased activity of dextran hydrolysis Arthrobacter globiformis
3.2.1.94 D227N decreased activity of dextran hydrolysis Arthrobacter globiformis
3.2.1.94 D227Q decreased activity of dextran hydrolysis Arthrobacter globiformis
3.2.1.94 D295A activity similar to wild-type enzyme Arthrobacter globiformis
3.2.1.94 D340A activity similar to wild-type enzyme Arthrobacter globiformis
3.2.1.94 D342A no activity of dextran hydrolysis Arthrobacter globiformis
3.2.1.94 D342E decreased activity of dextran hydrolysis Arthrobacter globiformis
3.2.1.94 D342N decreased activity of dextran hydrolysis Arthrobacter globiformis
3.2.1.94 D342Q decreased activity of dextran hydrolysis Arthrobacter globiformis
3.2.1.94 D373A activity similar to wild-type enzyme Arthrobacter globiformis
3.2.1.94 D396A activity similar to wild-type enzyme Arthrobacter globiformis
3.2.1.94 E420A activity similar to wild-type enzyme Arthrobacter globiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.94 65000
-
 SDS-PAGE Arthrobacter globiformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.94 Arthrobacter globiformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.94 90% purity Arthrobacter globiformis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.94 isomaltotriose + H2O = alpha-isomaltose + D-glucose mechanism Arthrobacter globiformis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.94 0.012
-
 E420A mutant Arthrobacter globiformis
3.2.1.94 0.17
-
 D396A mutant Arthrobacter globiformis
3.2.1.94 0.22
-
 D340A mutant Arthrobacter globiformis
3.2.1.94 0.24
-
 D373A mutant Arthrobacter globiformis
3.2.1.94 0.35
-
 D295A mutant Arthrobacter globiformis
3.2.1.94 0.45
-
 D163A mutant Arthrobacter globiformis
3.2.1.94 0.67
-
 D107A mutant Arthrobacter globiformis
3.2.1.94 0.78
-
 wild-type enzyme Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.94 dextran + H2O
-
 Arthrobacter globiformis alpha-isomaltose + O-alpha-D-Glcp-(1-3)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-4)-O-alpha-D-Glcp-(1-6)-D-Glc + O-alpha-D-Glcp-(1-2)-O-alpha-D-Glcp-(1-6)-D-Glc
-
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