Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fu, R.H.; Wang, Y.L.; Sung, H.Y.
    Cloning, characterization and functional expression of a new beta-D-fructofuranosidase (Osbetafruct2) cDNA from Oryza sativa (2003), Biotechnol. Lett., 25, 455-459.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.26 expression in Pichia pastoris Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 66000
-
 gel filtration Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Oryza sativa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.26 additional information the amino acid sequence contains four potential N-linked glycosylation sites Oryza sativa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 recombinant enzyme containing a 6*His-tagged tail Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 raffinose + H2O
-
 Oryza sativa beta-D-fructose + alpha-D-melibiose
-
 ?
3.2.1.26 sucrose + H2O
-
 Oryza sativa beta-D-fructose + alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 monomer 1 * 66000, SDS-PAGE Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 40
-
-
 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 4.8
-
-
 Oryza sativa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.26 Oryza sativa calculation from nucleotide sequence
-
 4.9