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Literature summary extracted from

  • Koh, H.; Igarashi, S.; Sode, K.
    Surface charge engineering of PQQ glucose dehydrogenase for downstream processing (2003), Biotechnol. Lett., 25, 1695-1701.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.5.2 biotechnology surface charge engineering of the enzyme for optimization of downstream processing in large scale enzyme production Acinetobacter calcoaceticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 expression in the periplasm of Escherichia coli strain PP2418, with and without a C-terminal tag of 3 Arg residues Acinetobacter calcoaceticus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.2 Q209R/N240R/T389R site-directed mutagenesis, increased thermal stability compared to the wild-type enzyme Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 additional information
-
 additional information kinetics Acinetobacter calcoaceticus
1.1.5.2 2
-
 D-galactose recombinant wild-type enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 4
-
 D-galactose recombinant tagged enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 5
-
 D-galactose recombinant triple mutant enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 10
-
 maltose recombinant wild-type enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 11
-
 maltose recombinant tagged enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 13
-
 maltose recombinant triple mutant enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 25
-
 D-glucose recombinant triple mutant enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 25
-
 lactose recombinant wild-type enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 26
-
 lactose recombinant tagged enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 27
-
 D-glucose recombinant wild-type enzyme and tagged enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 36
-
 lactose recombinant triple mutant enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 41
-
 3-O-methyl-D-glucose recombinant wild-type enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 53
-
 3-O-methyl-D-glucose recombinant tagged enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 56
-
 3-O-methyl-D-glucose recombinant triple mutant enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 63
-
 allose recombinant wild-type enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 75
-
 allose recombinant tagged enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus
1.1.5.2 76
-
 allose recombinant triple mutant enzyme, pH 7.0, 25°C Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.2 Ca2+ required Acinetobacter calcoaceticus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Acinetobacter calcoaceticus P13650
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2 optimization of the purification process involving cation exchange chromatography in presence of Zn2+ Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 3-O-methyl-D-glucose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 allose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.5.2 D-galactose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus D-galactono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 lactose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.5.2 maltose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 PQQ glucose dehydrogenase
-
 Acinetobacter calcoaceticus
1.1.5.2 PQQGDH
-
 Acinetobacter calcoaceticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.2 25
-
 assay at Acinetobacter calcoaceticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.2 55
-
 recombinant tagged wild-type enzyme and mutant enzyme show about 90% remaining activity after 10 min, the nontagged wild-type enzyme shows about 40% remaining activity after 10 min Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 7
-
 assay at Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone i.e. PQQ Acinetobacter calcoaceticus