BRENDA - Enzyme Database

Characterization and regulation of NADP+-isocitrate dehydrogenase from Saccharopolyspora erythraea

Alvarado, A.; Flores, M.E.; Biotechnol. Lett. 25, 1175-1178 (2003)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
60% inhibition at 1 mM
Saccharopolyspora erythraea
1.1.1.42
ADP
20% inhibition at 1 mM
Saccharopolyspora erythraea
1.1.1.42
ATP
50% inhibition at 1 mM
Saccharopolyspora erythraea
1.1.1.42
Citric acid
50% inhibition at 1 mM
Saccharopolyspora erythraea
1.1.1.42
glyoxalate
20% inhibition at 1 mM, presence of 1 mM oxaloacetate results in 50% inhibition
Saccharopolyspora erythraea
1.1.1.42
GTP
10% inhibition at 1 mM
Saccharopolyspora erythraea
1.1.1.42
additional information
no inhibition by phosphoenolpyruvate, fructose 1,6-bisphosphate, and pyruvate
Saccharopolyspora erythraea
1.1.1.42
oxaloacetate
25% inhibition at 1 mM, presence of 1 mM glyoxalate results in 50% inhibition
Saccharopolyspora erythraea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.000028
-
NADP+
pH 7.5, 30°C
Saccharopolyspora erythraea
1.1.1.42
0.00013
-
DL-isocitrate
pH 7.5, 30°C
Saccharopolyspora erythraea
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
requirement for divalent cations
Saccharopolyspora erythraea
1.1.1.42
Mn2+
requirement for divalent cations, preferred metal ion
Saccharopolyspora erythraea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
60000
-
2 * 60000, SDS-PAGE
Saccharopolyspora erythraea
1.1.1.42
130000
-
native PAGE
Saccharopolyspora erythraea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Saccharopolyspora erythraea
-
CA340
-
1.1.1.42
Saccharopolyspora erythraea CA340
-
CA340
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
additional information
growth-associated specific activity profile, dextrose or fructose are preferred before lactose as sole carbon source
Saccharopolyspora erythraea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
additional information
-
activity in cell free enzyme extracts of cells grown on diffeent carbon sources
Saccharopolyspora erythraea
1.1.1.42
0.75
-
cell free enzyme extracts of cells grown on glucose, best carbon source
Saccharopolyspora erythraea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
DL-isocitrate + NADP+
-
655094
Saccharopolyspora erythraea
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
DL-isocitrate + NADP+
-
655094
Saccharopolyspora erythraea CA340
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
2 * 60000, SDS-PAGE
Saccharopolyspora erythraea
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
30
-
-
Saccharopolyspora erythraea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
-
Saccharopolyspora erythraea
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
preferred cofactor
Saccharopolyspora erythraea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
preferred cofactor
Saccharopolyspora erythraea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
2-oxoglutarate
60% inhibition at 1 mM
Saccharopolyspora erythraea
1.1.1.42
ADP
20% inhibition at 1 mM
Saccharopolyspora erythraea
1.1.1.42
ATP
50% inhibition at 1 mM
Saccharopolyspora erythraea
1.1.1.42
Citric acid
50% inhibition at 1 mM
Saccharopolyspora erythraea
1.1.1.42
glyoxalate
20% inhibition at 1 mM, presence of 1 mM oxaloacetate results in 50% inhibition
Saccharopolyspora erythraea
1.1.1.42
GTP
10% inhibition at 1 mM
Saccharopolyspora erythraea
1.1.1.42
additional information
no inhibition by phosphoenolpyruvate, fructose 1,6-bisphosphate, and pyruvate
Saccharopolyspora erythraea
1.1.1.42
oxaloacetate
25% inhibition at 1 mM, presence of 1 mM glyoxalate results in 50% inhibition
Saccharopolyspora erythraea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.000028
-
NADP+
pH 7.5, 30°C
Saccharopolyspora erythraea
1.1.1.42
0.00013
-
DL-isocitrate
pH 7.5, 30°C
Saccharopolyspora erythraea
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
requirement for divalent cations
Saccharopolyspora erythraea
1.1.1.42
Mn2+
requirement for divalent cations, preferred metal ion
Saccharopolyspora erythraea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
60000
-
2 * 60000, SDS-PAGE
Saccharopolyspora erythraea
1.1.1.42
130000
-
native PAGE
Saccharopolyspora erythraea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
additional information
growth-associated specific activity profile, dextrose or fructose are preferred before lactose as sole carbon source
Saccharopolyspora erythraea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
additional information
-
activity in cell free enzyme extracts of cells grown on diffeent carbon sources
Saccharopolyspora erythraea
1.1.1.42
0.75
-
cell free enzyme extracts of cells grown on glucose, best carbon source
Saccharopolyspora erythraea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
DL-isocitrate + NADP+
-
655094
Saccharopolyspora erythraea
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
DL-isocitrate + NADP+
-
655094
Saccharopolyspora erythraea CA340
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
2 * 60000, SDS-PAGE
Saccharopolyspora erythraea
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
30
-
-
Saccharopolyspora erythraea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
-
Saccharopolyspora erythraea