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Literature summary extracted from

  • Clemente-Jimenez, J.M.; Martinez-Rogriguez, S.; Mingorance-Cazorla, L.; de la Escalera-Hueso, S.; las Heras-Vazquez, F.J.; Rodriguez-Vico, F.
    Catalytic analysis of a recombinant D-hydantoinase from Agrobacterium tumefaciens (2003), Biotechnol. Lett., 25, 1067-1073.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.2
-
 Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 EDTA 70 mM Agrobacterium tumefaciens
3.5.2.2 Hg2+
-
 Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.2 0.074
-
 DL-hydroxyphenylhydantoin
-
 Agrobacterium tumefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Mn2+ stimulates Agrobacterium tumefaciens
3.5.2.2 Ni2+ stimulates Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Agrobacterium tumefaciens
-
 BQL9
-
3.5.2.2 Agrobacterium tumefaciens BQL9
-
 BQL9
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 dihydrouracil + H2O
-
 Agrobacterium tumefaciens ureidopropionate
-
 ?
3.5.2.2 dihydrouracil + H2O
-
 Agrobacterium tumefaciens BQL9 ureidopropionate
-
 ?
3.5.2.2 DL-hydroxyphenylhydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl-D-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-hydroxyphenylhydantoin + H2O
-
 Agrobacterium tumefaciens BQL9 N-carbamoyl-D-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-isoproylhydantoin + H2O
-
 Agrobacterium tumefaciens N-carbamoyl-D-valine
-
 ?
3.5.2.2 DL-isoproylhydantoin + H2O
-
 Agrobacterium tumefaciens BQL9 N-carbamoyl-D-valine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium tumefaciens hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium tumefaciens BQL9 hydantoic acid
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.2 30
-
-
 Agrobacterium tumefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.2 30
-
 30 min, about 75% of the activity that is retained after treatment for 30 min at 4°C Agrobacterium tumefaciens
3.5.2.2 40
-
 30 min, about 65% of the activity that is retained after treatment for 30 min at 4°C Agrobacterium tumefaciens
3.5.2.2 50
-
 30 min, about 45% of the activity that is retained after treatment for 30 min at 4°C Agrobacterium tumefaciens
3.5.2.2 60
-
 30 min, about 35% of the activity that is retained after treatmet for 30 min at 4°C Agrobacterium tumefaciens
3.5.2.2 70
-
 30 min, about 20% of the activity that is retained after treatment for 30 min at 4°C Agrobacterium tumefaciens
3.5.2.2 80
-
 30 min, about 15% of the activity that is retained after treatment for 30 min at 4°C Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 8.7 9.7 reaction with dihydrouracil Agrobacterium tumefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.2 8 9.7 pH 8.0: about 55% of maximal activity, pH 9.7: about 90% of maximal activity Agrobacterium tumefaciens