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Literature summary extracted from

  • Krasotkina, J.; Borisova, A.A.; Gervaziev, Y.V.; Sokolov, N.N.
    One-step purification and kinetic properties of the recombinant L-asparaginase from Erwinia carotovora (2004), Biotechnol. Appl. Biochem., 39, 215-221.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.1 medicine a prospective therapeutic enzyme for leukemia treatment Pectobacterium carotovorum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expression in Escherichia coli Pectobacterium carotovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.00016
-
 L-Asn 37°C Pectobacterium carotovorum
3.5.1.1 0.442
-
 L-aspartyl-beta-hydroxamate 37°C Pectobacterium carotovorum
3.5.1.1 0.832
-
 D-Asn 37°C Pectobacterium carotovorum
3.5.1.1 3.44
-
 L-Gln 37°C Pectobacterium carotovorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 34000
-
 x * 34000, SDS-PAGE Pectobacterium carotovorum

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Pectobacterium carotovorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Pectobacterium carotovorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 630
-
-
 Pectobacterium carotovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 D-Asn + H2O
-
 Pectobacterium carotovorum D-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Pectobacterium carotovorum L-Asp + NH3
-
 ?
3.5.1.1 L-aspartyl-beta-hydroxamate + H2O
-
 Pectobacterium carotovorum L-Asp + hydroxylamine
-
 ?
3.5.1.1 L-Gln + H2O
-
 Pectobacterium carotovorum L-Glu + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 ? x * 34000, SDS-PAGE Pectobacterium carotovorum

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 ECAR-LANS
-
 Pectobacterium carotovorum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 42
-
 L-Gln 37°C Pectobacterium carotovorum
3.5.1.1 68
-
 D-Asn 37°C Pectobacterium carotovorum
3.5.1.1 450
-
 L-aspartyl-beta-hydroxamate 37°C Pectobacterium carotovorum
3.5.1.1 1600
-
 L-Asn 37°C Pectobacterium carotovorum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 6.5 9 pH 6.5: about 70% of the ativity at pH 9.0, pH 9.0: activity increases from pH 5.5 to pH 9.0 Pectobacterium carotovorum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.1 Pectobacterium carotovorum isoelectric-focusing, pH-gradient 3.5-9.5
-
 8.5