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Literature summary extracted from

  • Batra, N.; Singh, J.; Banerjee, U.C.; Patnaik, P.R.; Sobti, R.C.
    Production and characterization of a thermostable beta-galactosidase from Bacillus coagulans RCS3 (2002), Biotechnol. Appl. Biochem., 36, 1-6.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.23 EDTA 20.0 mM, activation to 116% Weizmannia coagulans

Application

EC Number Application Comment Organism
3.2.1.23 nutrition high thermostability and pH-stability and good hydrolytic capability make this enzyme potentially useful in dairy industry Weizmannia coagulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cu2+ 2.0 mM, 91% inhibition Weizmannia coagulans
3.2.1.23 D-glucose 0.1 M, 12% inhibition Weizmannia coagulans
3.2.1.23 galactose 0.1 M, 91% inhibition, competitive Weizmannia coagulans
3.2.1.23 Hg2+ 2.0 mM, 66% inhibition Weizmannia coagulans
3.2.1.23 Ni2+ 2.0 mM, 76% inhibition Weizmannia coagulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Weizmannia coagulans
-
-
-
3.2.1.23 Weizmannia coagulans RCS3
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Weizmannia coagulans D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Weizmannia coagulans RCS3 D-glucose + D-galactose
-
 ?
3.2.1.23 additional information efficient hydrolysis of whey Weizmannia coagulans ?
-
 ?
3.2.1.23 additional information efficient hydrolysis of whey Weizmannia coagulans RCS3 ?
-
 ?
3.2.1.23 o-nitrophenyl beta-D-galactopyranoside + H2O
-
 Weizmannia coagulans o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl beta-D-galactopyranoside + H2O
-
 Weizmannia coagulans RCS3 o-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 65
-
-
 Weizmannia coagulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 50 70 50°C: about 45% of maximal activity, 70°C: about 30% of maximal activity Weizmannia coagulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 63
-
 half-life: 15 h Weizmannia coagulans
3.2.1.23 65
-
 half-life: 2 h Weizmannia coagulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.8
-
-
 Weizmannia coagulans